The present invention deals with a new family of Dye Doped Silica Nanoparticles (DDSNPs), showing distinctive improvements when involved in electrochemiluminescent-based analysis.
| Patent title | Nanoparticles to improve analytical signal |
|---|---|
| Thematic area | Health |
| Ownership | ALMA MATER STUDIORUM - UNIVERSITA' DI BOLOGNA |
| Inventors | Enrico Rampazzo, Francesco Paolucci, Luca Prodi, Massimo Marcaccio, Damiano Genovese, Giovanni Valenti |
| Protection | International (PCT) |
| Licensing status | Available for development agreements, option, license and other exploitation agreements |
| Keywords | Dye doped silica nanoparticle, Electrochemiluminescence, Analytical methods, Biomarker, Clinical analysis, Optical transduction |
| Filed on | 09 September 2020 |
The quantification of diagnostic markers, or biomarkers, has an enormous impact in the early diagnosis, from basic research to clinical applications. In this context, electrochemiluminescence (ECL) appears to be a leading transduction technique for the detection of very low amounts of these molecules. ECL boasts also excellent spatial and temporal control with the possibility of performing rapid measurements on small sample volumes. Furthermore, the device for generating and reading the signal can be very simple, low cost and small in size, allowing the design of Point-of-care devices, and its use by non-specialized personnel for massive screening operations.
Chasing an ever-increasing sensitivity, many researchers tried to combine ECL with nanomaterials, such as nanoparticles, using them as dyes or co-reactants. In particular, dye-doped silica nanoparticles (DDSNPs) have proved to be a very interesting option as ECL dyes, since they can offer several advantages in terms of sensitivity and performance.
The present invention deals with a new family of Dye Doped Silica Nanoparticles (DDSNPs), showing distinctive improvements when involved in electrochemiluminescent-based analysis.
The synthetic approach herein adopted allows a high dye doping degree (ca 4800 complexes every NP), with limited variations of the NP surface charge, thus allowing a high ECL emission. Moreover, the silica matrix is able to increase the time stability of the ECL signal, increasing even more the performance of these NPs. Such results open a new promising path towards more sensitive analyte detection in biosensing and in point-of-care devices.
Application:
- Immunoassay-based detection systems
Advantages:
- Enhancement of signal intensity
- Simple and versatile synthetic schemes for the preparation.
- Enhancement of stability
Zanut, A., Palomba, F., Rossi Scota, M., Rebeccani, S., Marcaccio, M., Genovese, D., Rampazzo, E., Valenti, G., Paolucci, F., Prodi, L., Dye-Doped Silica Nanoparticles for Enhanced ECL-Based Immunoassay Analytical Performance, (2020) Angewandte Chemie - International Edition, 59 (49), pp. 21858-21863.