B2915 - Supramolecular Chemistry Techniques and Methods for the Study of Drugs

Learning outcomes

The goal of the course is to provide the industrial pharmacist with the concepts and experimental tools used to investigate the supramolecular phenomena involved in drug/molecular target interactions. The student will familiarize with nucleic acids and proteins chemistry, the chemical and physical factors ruling supramolecular processes, the techniques used in supramolecular investigation.

Course contents

Supramolecular chemistry concepts: molecular recognition, cooperativity, self-assembly and self-organization.

Determination of binding constants: UV-Vis and NMR methods.

Calorimetric titrations (ITC).

NOE and diffusion NMR (DOSY) and their applications in supramolecular chemistry.

Circular dichroism for the investigation of biomolecules in solution and their supramolecular behaviour.

Aminoacids, peptides, proteins, nucleotides and oligonucleotides: preparation and supramolecular behaviour.

Nucleic acids and proteins sequencing.

Peptides and oligonucleotides as drugs: selected examples.

Readings/Bibliography

• J. W. Steed, J. L. Atwood - Supramolecular Chemistry-Wiley (2022)
• C.A. Schalley (ed.) Analytical Methods in Supramolecular Chemistry II ed. (2012) WILEY-VCH (D)
• T. D. W. Claridge - High-resolution NMR techniques in organic chemistry- II ed. (2009) Elsevier
• Blackburn G.M. e Gait, M.J. (eds.) Nucleic Acids in Chemistry and Biology II ed., (1996) o subsequent, Oxford University Press (UK).
• Gait, M.J. (ed.) Oligonucleotide Synthesis a Practical Approach (1984) IRL Press Oxford (UK).
• J. Jones, Aminoacid and peptide synthesis, Oxford University Press, 1992.
• M. Bodanszki, Principles of peptide synthesis, 2nd ed. Springer, 1993.

Teaching methods

Lectures

Teaching tools

Presentations. Files  will be made available for download.

Office hours

See the website of Stefano Masiero