90171 - Biology and Genetics Lab-based Course (B)

Learning outcomes

At the end of the course, the student has experienced the main methods of cell biology and general and molecular genetics applied to biomedical sciences. In particular, it knows the fields of application and the limits of the main techniques used in cell biology, with particular attention to cell cultures, biotechnologies and the use of transgenic animals. He has learned to recognise the elements of a gene in a real DNA sequence, is able to consult the major online molecular genetic banks; he has performed a simple DNA extraction, and has discussed the rational basis of the method; he has designed an experiment of polymerase chain reaction (PCR) to amplify DNA. He has learned to reconstruct the path from experimental data to building current biomedical knowledge to evaluate the methodological aspects of knowledge by taking a critical approach to the study.

Course contents

The Laboratory of Biology and Genetics is organized in theoretical-practical activities aimed at examining several aspects of the translational medicine: from research to clinical application. These activities are complementary to the frontal lessons and are designed to create an integrated path between Biology and Genetics, shared by the teachers. The student will have the opportunity to approach the theoretical topics, addressed in classroom, from an experimental point of view. The student will focus the attention on some molecular and cellular biology technologies that have led to fundamental biological-genetic discoveries and which continue to be used in the biomedical field, understanding the importance of these technologies in the basic sciences and in the translational medicine. The student will also use multi-media systems and will learn to use software and databases useful for solving the biological problems posed.

Readings/Bibliography

Protocols, articles and schemes from medical journals provided by the teacher.

Teaching methods

- Virtual experiences, movies, teamwork.

- Use of databases.

- Practical laboratory experiences.

As concerns the teaching methods of this course unit, all students must attend Module 1, 2 on Health and Safety online.

Assessment methods

Discussion at the end of each activity. In the final examination, a discussion of theoretical-practical activities may be required.

Teaching tools

- Slides with images and schemes.

- Databases and tutorials available online and softwares.

- Scientific articles.

Office hours

See the website of Francesca Antonaros