- Docente: Ilaria Braschi
- Credits: 2
- Language: Italian
- Moduli: Ilaria Braschi (Modulo Mod 1) Stefano Tartarini (Modulo Mod 2)
- Teaching Mode: Traditional lectures (Modulo Mod 1) Traditional lectures (Modulo Mod 2)
- Campus: Bologna
- Corso: Second cycle degree programme (LM) in Agricultural Sciences and Technologies (cod. 9235)
Learning outcomes
Once the programme is completed, students will have acquired basic knowledge on the analytical methodologies and skills to correctly operate analytical instruments usually adopted in the field of plant production and biotechnologies. By approaching specific case studies, students will be invited to manage and develop innovative plant production systems.
Course contents
Module 1 (25 hours)
1. Classroom teaching (15 hours)
Plant analysis Theory (10 hours)
1.1. Liquid and gas chromatographic analysis: Theory and relevance.
1.2. PCR quantitative real time: Theory and relevance.
1.3. Leaf gas exchanges and chlorophyll fluorescence
1.4. Digital PCR: from relative to absolute quantification.
2. Lab analytical trials (10 hours)
2.1. Chromatographical analysis of plant material
2.2. Preparation of nucleic acid extracts from plant tissues and real time quantitative PCR
2.3. Measurement of photosynthesis, transpiration and chlorophyll fluorescence.
2.4.Droplet digital PCR system (Bio-Rad QX200)
Module 2 (25 hours)
Post-harvest of horticultural products and flowers
Teaching Unit 1 (4 hours)
• Post-harvest physiology in fruit tree species
Teaching Unit 2 (4 hours)
• Post harvest of vebetable crops and fresh cut products
Teaching Unit 3 (4 hours)
• Post-harvest physiology of flowers
Teaching Unit 4 (4 hours)
• Conditioning technologies and distribution of horticultural products
Technical Visits (9 hours)
Readings/Bibliography
- periodic table of the elements
- official methods of soil analysis available on the web
- slides provided by theinstructor
- online manuals
Teaching methods
- During the classroom teaching, students will acquire knowledge of the main techniques of analysis of soil and plant material. The knowledge acquisition as well as the comprehension ability of students will be monitored along the entire teaching period through a continuous interactions between the teacher and students through the problem solving approach. The teacher-student interaction is also finalized to improve the development of the evaluation skills and to increase the communication ability of the students.
- Owing to the high number of students attending the course that limit their admission to the laboratories of the Department of Agri-Food Sciences and Technologies, the analytical trails will be operated remotely and safely from the class by the software teamviewer
Assessment methods
The assessment of each teaching module will be evaluated through a qualifying written examination made of 30 multiple answer questions. The final assessment of each module will be successfully passed with 18 correct answers out of 30.
Teaching tools
Blackboard
PC
Projector
software TeamViewer
Isotopic mass spectrometer
HPLC-Diodarray
LC-MS
Real time quantitative PCR thermalcycler
LiCor 6400 for gas exchange and fluorescence
Office hours
See the website of Ilaria Braschi
See the website of Stefano Tartarini