Abstract
The ESILARANTE project aims to develop an innovative portable biosensing platform for the detection of cannabinoid in capillary blood samples, based on laccase biosensors fabricated via Electrospray Deposition (ESD), a green and cost-effective enzyme immobilization technique. The project combines enzyme purification, physico-chemical characterization and advanced electrochemical testing to identify the factors enabling high sensor stability and reusability. Particular attention is given to the analytical performance, with quantitative evaluation of enzymatic activity and validation against fully validated LC-MS/MS protocols. Analytical techniques also investigate enzyme integrity before and after deposition, helping to optimize the system. The expected result is a robust, selective, and miniaturized biosensor for rapid, on-site applications in forensic and roadside settings, representing a sustainable and scalable alternative to conventional laboratory-based cannabinoid testing.
Dettagli del progetto
Responsabile scientifico: Michele Protti
Strutture Unibo coinvolte:
Dipartimento di Farmacia e Biotecnologie
Coordinatore:
CNR - Consiglio Nazionale delle Ricerche(Italy)
Contributo totale Unibo: Euro (EUR) 79.000,00
Durata del progetto in mesi: 24
Data di inizio
28/09/2023
Data di fine:
28/02/2026
