Abstract
White adipose tissue browning promotes type 2 diabetes (T2D) control. A gut microbiome (GM)-adipose tissue axis is involved, but the mechanisms are unclear. This study aims to demonstrate the causal role of human GM in browning in a T2D model and unveil underlying mechanisms, and to identify the molecules that promote browning. T2D mice will undergo GM depletion by antibiotics with or without fecal microbiota transplantation from children with highly active brown adipose tissue. The following will be measured: body weight, food intake, glucose/insulin tolerance, glucose metabolism and adipose depot density (by PET/CT imaging), browning, intestinal wall morphology and hypothalamic inflammation (by histology). Metabolomics and molecular analyses of blood and adipose tissue, and GM profiling will allow identifying involved taxa, functional pathways and molecules. Selected molecules will be tested in vitro on adipose tissue explants from T2D mice to identify new therapeutic leads.
Dettagli del progetto
Responsabile scientifico: Silvia Turroni
Strutture Unibo coinvolte:
Dipartimento di Farmacia e Biotecnologie
Coordinatore:
CNR - Consiglio Nazionale delle Ricerche(Italy)
Contributo totale Unibo: Euro (EUR) 46.603,00
Durata del progetto in mesi: 24
Data di inizio
18/10/2023
Data di fine:
28/02/2026
