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90929 - Classical and Next-Generation Genomics

Academic Year 2022/2023

  • Moduli: Pierluigi Strippoli (Modulo 1) Pierluigi Strippoli (Modulo 2)
  • Teaching Mode: Traditional lectures (Modulo 1) Traditional lectures (Modulo 2)
  • Campus: Bologna
  • Corso: Second cycle degree programme (LM) in Medical Biotechnology (cod. 9081)

Learning outcomes

Discuss the structure, organization, variability, genetic information and gene families of the human genome. Critically appraise the rational basis of the main molecular and computational biology methods used for the study of genomes, including both classical and next-generation approaches. Consult and study bibliographic sources to self-update on innovative genomic topics and techniques. Use common software tools for genetics and genomics, and apply them to the biomedical field.

Course contents

1. Human Genomics and Post-Genomics

2. Bibliography and Genomics databases

3. Methods for studying nucleic acid

4. Nucleic Acids Hybridization (Southern Blot, Northern Blot, Microarray)

5. Polymerase Chain Reaction (Kinetics, Parameters, Design)

6. cDNA, EST and genomic Libraries

7. Nucleic Acids Sequencing and Sequence Analysis

8. Computational and System Biology - Trisomy 21

Laboratory (15 hours)

1. Advanced use of bibliography and genomics databanks

2. Gene structure - NCBI Gene database

3. PCR primer design

4. Sequence analysis

5. Documentary and discussion

Readings/Bibliography

Website: http://apollo11.isto.unibo.it/

Website: http://apollo11.isto.unibo.it/biotech/en/Biotech.htm

Teaching materials will be made available through both public websites and Student access to the "Virtuale" platform.

Teaching methods

In consideration of the type of activity and the teaching methods adopted, the attendance of this training activity REQUIRES the prior participation of all Students in the Training Modules 1 and 2 about safety in the study places, in e-learning mode.

Assessment methods

16 multiple choice questions on the treated topics, in person. Attendance to learning activities is mandatory; the minimum attendance requirement to be admitted to the final exam is 60% of lessons and laboratories.

Teaching tools

Availability of personal computers (iMac) in the "Auletta Multimediale".

Office hours

See the website of Pierluigi Strippoli [https://www.unibo.it/sitoweb/pierluigi.strippoli/en]

Office hours

See the website of Pierluigi Strippoli