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42874 - Genomics

Academic Year 2018/2019

                
                        Docente:
                        Pierluigi Strippoli
                    
                        Credits:
                        4
                    
                        SSD:
                        BIO/13
                    
                        Language:
                        Italian
                    
                        Teaching Mode:
                        Traditional lectures
                        
                            Campus:
                            Bologna
                        
                            Corso:
                            Second cycle degree programme (LM) in
                            Medical Biotechnology (cod. 8859)

                            Teaching resources on Virtuale

Course contents

REGENERATIVE MEDICINE AND GENOMICS (C.I.) A.A. 2018/2019

GENOMICS

Auletta Multimediale, Dipartimento di Medicina Specialistica, Diagnostica e Sperimentale,

Sede Operativa di Istologia, Embriologia e Biologia Applicata, Via Belmeloro 8, 40126 Bologna (BO).

Attending 70% of the lessons/laboratories is required.

Lessons (24 hours)

rational background of common methods in Genomics

1. Human Genomics and Post-Genomics [WED OCTOBER 10, 2018, 14:30-17:00]

2. Bibliography and Genomics databases [WED OCTOBER 17, 2018, 14:00-16:30]

3. Methods for studying nucleic acids (Extraction, Electrophoresis, Enzymes) [THU OCTOBER 18, 2018, 14:00-16:30]

4. Nucleic Acids Hybridization (Southern Blot, Northern Blot, Microarray) [WED OCTOBER 24, 2018, 14:00-16:30]

5. Polymerase Chain Reaction (Kinetics, Parameters, Design) [THU NOVEMBER 08, 2018, 14:00-16:30]

6. cDNA, EST and genomic Libraries [TUE NOVEMBER 13, 2018, 14:00-16:30]

7. Nucleic Acids Sequencing and Sequence Analysis [THU NOVEMBER 15, 2017, 14:00-16:30]

8. Computational and System Biology - Trisomy 21 [WED NOVEMBER 28, 2018, 16:00-18:30]

Laboratory (15 hours)

1. Advanced use of bibliography and genomics databanks [TUE OCTOBER 23, 2018, 14:00-16:30]

2. Gene structure - NCBI Gene database [WED OCTOBER 31, 2018, 14:00-16:30]

3. PCR primer design [FRI NOVEMBER 09, 2018, 14:00-16:30]

4. Sequence analysis [TUE NOVEMBER 20, 2018, 14:00-16:30]

5. Documentary and discussion [THU NOVEMBER 29, 2018, 16:00-18:30]

Readings/Bibliography

Fantoni A. et al. Genetica. Piccin, 2009 (Chapter 33: "Human Genome Analysis" - in Italian).

Sito: http://apollo11.isto.unibo.it/

Assessment methods

Short written essay, to be sent as a file via e-mail within the day of the examination.

Teaching tools

Availability of 19 personal computers (iMac) in the "Auletta Multimediale".

Office hours

See the website of Pierluigi Strippoli