14041 - Biochemistry Lab-based Course (L-Z 1)

Academic Year 2023/2024

  • Teaching Mode: Traditional lectures
  • Campus: Bologna
  • Corso: Single cycle degree programme (LMCU) in Medicine and Surgery (cod. 5904)

Learning outcomes

At the end of the module, the student acquires methodological and applicative elements useful for integrating the knowledge obtained in the courses of Chemistry and Biochemistry.

Course contents

CHEMISTRY LABORATORY

Behavior of ions in aqueous solution: a metallic ion, an amphoteric ion, transition metal ions.

Formation reactions of complex ions. Oxidation-reduction reactions.

pH indicators.

Acid-base titrations: strong acid-strong base titration with pH indicator; potentiometric titration (pH meter) weak acid-strong base. Choice of indicators and their coloration depending on pH. Processing of experimental data obtained. Determination of the dissociation constant of weak acids and bases (Ka, Kb). Composition and properties of a buffer solution.

Benedict's test for reducing sugars.


METABOLIC BIOCHEMISTRY LABORATORY

Study of the metabolic biochemistry program through the presentation of clinical cases.

Teaching methods

Practical exercises in small groups will be conducted after adequate explanation in lectures of the theoretical principles underlying the exercises, the use of laboratory materials, and safety regulations for handling products and equipment.


Considering the types of activities and teaching methods adopted, the attendance of this training activity requires all students of modules 1 and 2 to participate in e-learning mode [https://www.unibo.it/it/servizi-e-opportunita/salute-e-assistenza/salute-e-sicurezza/sicurezza-e-salute-nei-luoghi-di-studio-e-tirocinio] and attend module 3 specific training on safety and health in study places. Instructions on dates and attendance modalities for module 3 can be found in the appropriate section of the course's website.

Assessment methods

The laboratory activity contributes to achieving the educational objectives of the General Chemistry and Biochemistry module.


Students are required to submit the titration curve of acetic acid and the related calculations (acid concentration and Ka) using the "assignment" function on the Virtual platform (https://virtuale.unibo.it) before taking the General Chemistry and Biochemistry exam. The assignment will not be graded.

Office hours

See the website of Silvia Cetrullo