- Docente: Valentina Giorgio
- Credits: 1
- Language: Italian
- Teaching Mode: Traditional lectures
- Campus: Bologna
- Corso: Single cycle degree programme (LMCU) in Medicine and Surgery (cod. 5904)
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from Mar 11, 2024 to Apr 10, 2024
Learning outcomes
At the end of the laboratory the student will be aware of the basis of Biochemistry with focus on enzyme kinetics and bioenergetics.
Course contents
Biochemical laboratory
- Kinetics and enzymatic regulation. Quantitative and spectrophotometric analysis of enzymes and their characteristics. Calculation of Km and Vmax.
- Energetic coupling and ATP production of oxidative phosphorylation. Measuring of respiration in cells and isolated mitochondria through oxygraphy. Calculation of oxygen consumption rate in state 3 and 4. Determination of the energetic coupling level with V(state 3)/V(state 4). Determination of the efficiency in oxidative phosphorylation through the ATP/O ratio.
Teaching methods
Laboratory and bioinformatics activities.
Assessment methods
The acquired knowledge will be evaluated durin the oral exam.
Teaching tools
Dedicated softwares will be provided in the informatics rooms.
Office hours
See the website of Valentina Giorgio