- Docente: Claudia Zanna
- Credits: 1
- Language: Italian
- Teaching Mode: Traditional lectures
- Campus: Bologna
- Corso: Single cycle degree programme (LMCU) in Medicine and Surgery (cod. 5904)
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from Dec 06, 2023 to Dec 20, 2023
Learning outcomes
At the end of the module the student acquires methodological and applicative elements useful for integrating the knowledge gained in the teachings of Chemistry and Biochemistry.
Course contents
Molecular techniques used for qualitative and quantitative analysis of DNA, used in the fields of clinical diagnostics, forensic medicine, and research.
PCR and Real-time PCR, Gel electrophoresis, Southern blotting, DNA fingerprinting.
Applications of the indicated techniques. Exercises: kinship test, identification of suspects in forensic genetics, clinical diagnoses (Duchenne muscular dystrophy, Huntington; sickle cell anemia, Sars-COV2 positivity…). Use of dedicated software to design primers to amplify genes by PCR.
Readings/Bibliography
The material is provided to the students during each exercise
Teaching methods
The exercise is introduced by a brief theoretical presentation on principles, methods and techniques object of practical experience or by computer simulation.
Assessment methods
The evaluation will take place in the context of the overall evaluation of the C.I. and in particular during the end-of-year interview
Teaching tools
The exercises will be held in rooms equipped with computers
Office hours
See the website of Claudia Zanna