- Docente: Martina Cappelletti
- Credits: 6
- SSD: BIO/19
- Language: English
- Moduli: Marco Rinaldo Oggioni (Modulo 1) Martina Cappelletti (Modulo 2)
- Teaching Mode: Traditional lectures (Modulo 1) Traditional lectures (Modulo 2)
- Campus: Bologna
- Corso: First cycle degree programme (L) in Genomics (cod. 9211)
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from Sep 28, 2023 to Nov 10, 2023
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from Oct 23, 2023 to Nov 28, 2023
Learning outcomes
By the end of the module the student has knowledge of the main approaches for genomic data analysis and annotation in prokaryotes, with an emphasis on the use of next generation sequencing for the functional and structural analysis of genomes. In particular the student is able to: understand the structure of genetic variability and its phenotypic effects, browse prokaryotic genomes, apply methods for genomic data analysis, and correctly interpret and plan genomic studies in bacteria.
Course contents
The Prokaryotic Genomes course (Module1+Module2) will focus on the following topics:
- Introduction to Prokaryotes: the prokaryotic cell and microbial growth
- Diversity of Prokaryotic World: bacterial and archeal taxonomy and phylogeny, the tree of life, molecular chronometers and phylogenetic tree, diversity based on nutrition, metabolism and physiology
- Prokaryotic genomes and plasticity: genome structure and partitioning, bacterial plasmids and transposable elements, Genomic Islands, core genomes VS accessory genomes, bacterial conjugation, transduction and transformation, recombination
- Principles of mutagenesis, epigenetics and antimicrobial-drug resistance
- Genome sequencing: Genome sequencing technologies, Genome Reconstruction/assembly, Genome Annotation
- Comparative genomics of Prokaryotes and Microbial Genome Databases
- Functional Genomics: Transcriptomics (RNA-seq and Microarray)
- Introduction to CRISPR: the prokaryotic Adaptive Immune System and the genome editing tool
Readings/Bibliography
No specific text-books are required. The material used can be obtained on-line in the form of scientific reviews and/or specific publications in ISI journals.
The following are suggested books for additional insights into the course topics:
- "Bacterial Genetics and Genomics" by Lori A.S. Snyder (2020), CRC press Taylor and Francis Group
- Genomes 5 by T.A. Brown (2023), CRC press Taylor and Francis Group
Teaching methods
Topics in this course will be presented through frontal lectures by using detailed Power Point presentations that will be uploaded on Virtuale unibo to be available for the students.
Some lectures of the Module 2 will be practical and will be given in the bioinformatic teaching laboratory.
Assessment methods
The final Course examination consists of a written (short-answer, long-answer and multiple-choice questions).
Teaching tools
Lectures will be given with power-point presentations. Reviews and research publications relevant to the topics of the course will be provided at the end of each lesson along with additional teaching materials. Online open sourced video’s may also be assigned to complement the lectures.
Links to further information
https://site.unibo.it/molecular-environmental-microbiology-lab/it/teaching
Office hours
See the website of Martina Cappelletti
See the website of Marco Rinaldo Oggioni