- Docente: Eugenia Franchini
- Credits: 2
- Language: Italian
- Teaching Mode: Traditional lectures
- Campus: Bologna
- Corso: First cycle degree programme (L) in Biomedical Laboratory techniques (cod. 8484)
-
from Mar 04, 2024 to Apr 12, 2024
Learning outcomes
At the end of the course the student acquires the fundamental notions for the preparation and critical evaluation of the results of preparations for the cytohistopathological diagnostic investigation
Course contents
Introduction to the pathological anatomy laboratory: types of samples, workflow, traceability.
Methodological bases of histology and cytology: techniques, instruments, basic and special stains.
Extemporaneous intraoperative histological examination: special methods and applications (sentinel lymphonode in the freezer and by PCR).
In situ molecular pathology techniques: manual and automated immunohistochemistry; chromogenic and fluorescent in situ hybridization.
In vitro molecular pathology techniques: extraction and quantification of nucleic acids from archive tissues; PCR; real-time PCR; digital PCR; Sanger sequencing; NGS; molecular hybridization on a solid support.
Readings/Bibliography
Course teaching material, slides provided by the teacher
Teaching methods
Frontal lessons
Assessment methods
Written exam on the topics covered in the course
Teaching tools
Viewing of digitized histological preparations
Office hours
See the website of Eugenia Franchini