39314 - Immunohistochemistry

Learning outcomes

Upon completion of the course, the student possesses the fundamentals for the set-up and critical outcome evaluation of immunohistochemical preparations, as well as related internal quality controls. He or she also acquires the basic concepts for understanding the rationale for choosing reagents and target proteins (markers), in differential diagnosis and prognostic-predictive applications.

Course contents

Module 1 immunohistochemistry: Lectures and slides:

- Immunohistochemistry methods

- Use of automated platforms

- Tissue-derived markers and their interpretation in histologic diagnostics

- Predictive markers and their significance in the diagnosis of malignancies

- Immunohistochemical typing of metastatic processes

- Metastases of unknown origin

Module 2 Autopsy Techniques: Lecture and slides with access to the septic room for students who express interest in following a diagnostic finding. Times and days cannot be defined in advance for this activity as it is related to the departments' request for a diagnostic finding:

- Introduction with historical background;

- Difference between diagnostic finding and judicial autopsy and definitions;

- Autopsy techniques:

o when it is performed (Art 8 Mortuary Police Regulations);

o personnel involved and tools needed;

o cadaveric phenomena (abiotic and transformative) ;

o description of the external examination of the corpse;

o description of the internal examination of the corpse.

- In-depth study of myocardial infarction both macroscopically and microscopically;

- Insight of atherosclerosis at both macroscopic and microscopic level;

- In-depth macroscopic examination of the brain.

Readings/Bibliography

Course lectures are uploaded to "vituale" before each lessons.

Teaching methods

Discussion of the topics treated in the lessons

Office hours

See the website of Antonietta D'Errico