- Docente: Lucia Catani
- Credits: 3
- SSD: MED/15
- Language: English
- Moduli: Lucia Catani (Modulo 1) Lucia Catani (Modulo 2)
- Teaching Mode: Traditional lectures (Modulo 1) Traditional lectures (Modulo 2)
- Campus: Bologna
- Corso: Second cycle degree programme (LM) in Medical Biotechnology (cod. 9081)
Learning outcomes
Describe the biological properties of the hematopoietic stem cells and relevant biotechnological applications. Describe the phenotype and genotype of hematological malignancies. Discuss the principles of targeted therapy and cell therapy with particular reference to the transplantation of stem cells and regenerative medicine. Define the molecular targets and cellular structures suitable for the development of new forms of targeted therapy. Critically evaluate new diagnostic and therapeutic approaches in hematology.
Course contents
Master Degree in Medical Biotechnology
Course: First year
Course: Leukemic stem cells and hematological malignancies
Head of the course
Lucia Catani, PhD
Lessons place:
Aula Didattica Polo Murri -Policlinico Sant' Orsola
Programme
-Hemopoietic system and regulatory mechanisms
-Leukemic stem cells: bio-molecular and functional characterization
-Leukemic niche and the cross-talk with the leukemic stem cells
-Liquid biopsy assay: extracellular vesicles
-Acute Leukemia: classification, general concepts and bio-molecular/functional characterization
-Lymphoproliferative Diseases: classification, general concepts and bio-molecular/functional characterization
-Chronic Myeloproliferative Diseases: classification, general concepts and bio-molecular/functional characterization and "targeted therapy"
-Principles of cellular therapy: Hemopoietic stem cell transplantation; bone marrow microenvironment and hemopoietic stem cell mobilization
-Main experimental methods to characterize the hemopoietic stem/progenitor cells and cells of the bone marrow microenvironment
-How to design an experimental protocol and analyse results
By attending the associated laboratory, students will learn:
-methods to isolate the hemopoietic stem/progenitor cells
-methods to functionally characterize the isolated hemopoietic stem/progenitor cells
Readings/Bibliography
"Hematology-Pathophysiology, Diagnosis and Treatment" S. Tura, M. Cavo, PL. Zinzani-Società Editrice Esculapio Bologna. In addition, specific recent bibliography and web sites are suggested directly to students at the end of each lesson.
Teaching methods
The course includes frontal teaching and laboratory.
Frontal teaching include:
-slide presentation: Power-Point
-TED talks
In addition, the covered topics are implemented with experimental results deriving from ongoing research projects.
The laboratory garentees that each student is personally involved in carrying out the methods.
Assessment methods
Oral exam at the end of the course of lessons upon registration.
The oral exam includes 3 specific questions (the first chosen by the candidate and the others at the discretion of the teacher).
The final exam aims to evaluate the achievement of the educational objectives:
- To know the biomolecular bases of normal and leukemic hemopoiesis
- To understand the development processes of an experimental research plan and the evaluation of the results.
The final mark of the Integrated Course is defined on the average of the scores reported on topics related to the main objectives of the Course.
Teaching tools
Slides are directly available for students.
Office hours
See the website of Lucia Catani