- Docente: Luca Dondini
- Credits: 3
- SSD: AGR/03
- Language: English
- Teaching Mode: Traditional lectures
- Campus: Bologna
- Corso: Second cycle degree programme (LM) in International Horticultural Science (cod. 8883)
Learning outcomes
The course will provide the essential knowledge about the methods, application and the state of the art of plant and microbial biotechnologies including breeding for enhancing plant characteristics and fitness. A special focus will be paid on how plant-microbe interactions increase plant productivity, resistance and resilience to external stimuli Crop biotechnologies and molecular application will be studied. Students have to demonstrate a critical knowledge of the main goals and strategies of breeding in fruit tree aimed to release or select new genotypes for sustainable production
Course contents
Introduction to plant biotechnologies. In vitro culture. Embryorescue. In vitro morphogenesis and regeneration. Cell culture. Early selection in vitro. Somaclonal variation. Haploids. Main techniques of molecular marker analysis analysis in fruit tree species (RFLP, SCAR, CAPS, SSR, AFLP, SNP, functional markers). Applications of molecular markers in fruit tree species: identification of markers linked to monogenic traits, molecular maps, QTL analysis and polygenic traits, marker-assisted selection, identification of genes, genetic diversity. Genetic transformation in fruit trees and applications.
Readings/Bibliography
Handouts and selected papers
Teaching methods
Frontal lectures, lab experiments
Assessment methods
oral exam
Teaching tools
Beamer, equipments in the biotechnology lab
Office hours
See the website of Luca Dondini