- Docente: Marco Candela
- Credits: 3
- SSD: CHIM/11
- Language: English
- Teaching Mode: Traditional lectures
- Campus: Bologna
- Corso: Second cycle degree programme (LM) in Food Safety and Food Risk Management (cod. 9140)
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from Sep 23, 2022 to Dec 16, 2022
Learning outcomes
The successful student will know: (i) main goals of a metagenome study; (ii) techniques and technologies for the metagenomes assessment; (iii) role of microbiomes in food systems, food production and connections with food quality and the risk assessment.
Course contents
- General Introduction the study of microbiomes: metagenomics, microbial ecosystems and microbiomes, definitions, importance and applications, main technical aspects, goals for a metagenome study, types of metagenome studies, NGS sequencing, second-and third-generation NGS platforms;
- NGS sequencing: sampling, DNA extraction from different matrices (soil, air, water and biological samples), multiplexing and library preparation, NGS sequencing reaction; marker gene 16S sequencing and shotgun metagenomics;
- bioinformatic and biostatistics: demultiplexing, quality filtering, reads mapping, contigues reconstruction, assignment on reference databases (KEGG, COGS, greengenes and SILVA, alpha and beta diversity), multivariate statistics
- microbiomes in food system: microbiomes of the food system actors, microbiomes circulation in food systems and connection with food quality and safety, food system microbiome for a better food, microbiomes and risks assessment in foods and food production.
Readings/Bibliography
Lesk Introduction to Bioinformatics (V Edition_2019)
Metagenomics, perspectives, methods and applications, M Nagarajan (2017)
Brook, biology of microorganisms 15th edition
Notes of lectures; scientific publications
Teaching methods
Oral lectures and scientific seminaries
Assessment methods
Theoretical knowledge will be tested in a oral examination
Teaching tools
projector, computer, instruments of microbiological laboratory
Office hours
See the website of Marco Candela
SDGs
This teaching activity contributes to the achievement of the Sustainable Development Goals of the UN 2030 Agenda.