- Docente: Giorgio Gallinella
- Credits: 6
- SSD: MED/07
- Language: Italian
- Moduli: Giorgio Gallinella (Modulo 1) Giorgio Gallinella (Modulo 2)
- Teaching Mode: Traditional lectures (Modulo 1) Traditional lectures (Modulo 2)
- Campus: Bologna
- Corso: Second cycle degree programme (LM) in Pharmaceutical Biotechnology (cod. 8519)
Learning outcomes
After completing this module, students have advanced knowledge in the field of microbiology, with particular reference to the biodiversity of microorganisms and viruses, the dynamics of infectious processes and the rationale for developing vaccines. In particular, the student: - knows the main groups of microorganisms and viruses - knows the virulence and pathogenicity factors of microorganisms and viruses - knows the dynamics of disease processes and interactions with host - knows the strategies for the design and development of vaccines. Moreover, the student is familiar in the laboratory with the molecular and bioinformatic technologies necessary to the design and development of vaccines. In particular, the student is able to: - conduct bioinformatic analys of the genomes of microorganisms and viruses - use molecular biology technologies to produce recombinant baculoviruses for expression of heterologous protein.
Course contents
General principles for the development of vaccines.
Antiviral vaccines: inactivated virus, attenuated virus, subunit,
recombinant. Lines of biotechnological development of
vaccines.
Viruses of medical relevance and related vaccines: Picornaviridae,
Flaviviridae, togaviridae, Paramyxoviridae, Orthomyxoviridae,
Reoviridae, Hepadnaviridae, Papillomaviridae, Retroviridae,
Poxviridae.
Antibacterial vaccines: inactivated cells, attenuated
cells, toxoids, polysaccharides, protein
subunits.
Bacteria of medical relevance and related vaccines: Bacilli,
Staphylococci, Streptococci, Clostridia, Corynebacterium,
Mycobacterium, Neisseria, Enterobacteriaceae.
Reverse vaccinology: genomics, post-genomics and vaccine
development.
Laboratory module: expression of recombinant capsid protein of parvovirus B19 by using the baculovirus system.
Readings/Bibliography
Michele La Placa
Principi di Microbiologia Medica
Società Editrice Esculapio/EdiSES, 14a edizione (2014)
G.Antonelli, M.Clementi, G.Pozzi, G.M.Rossolini
Principi di Microbiologia Medica
Casa Editrice Ambrosiana, 2a edizione (2012)
Teaching methods
Lecture
Laboratory with supporting tutors
Assessment methods
The examination aims to assess the achievement of the
learning objectives of the module. Students are required to achieve
advanced knowledge in the field of microbiology, with particular
reference to the biodiversity of microorganisms and viruses, the
dynamics of infectious processes and the rational basis for the
development of vaccines. In particular:
- Knowledge of the main groups of microorganisms and viruses, their
genomic organization and their pathogenicity factors;
- Knowledge of the dynamics of infectious processes and
interactions with the host;
- Knowledge of strategies for the design and development of
vaccines;
- Ability to use molecular biology technologies for the production
of recombinant proteins and viruses.
The test consists of an interview on the program of the course, and
in the presentation and discussion of a report of the laboratory
experiments. The vote, out of thirty, will be given by the average
of the evaluation of the oral and the report presented.
Teaching tools
PowerPoint Presentations
Review articles on selected topics
Laboratory protocols
Office hours
See the website of Giorgio Gallinella