- Docente: Giulio Zanaroli
- Crediti formativi: 6
- SSD: CHIM/11
- Lingua di insegnamento: Inglese
- Modalità didattica: Convenzionale - Lezioni in presenza
- Campus: Bologna
- Corso: Laurea Magistrale in Low Carbon Technologies and Sustainable Chemistry (cod. 9246)
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dal 19/09/2024 al 20/12/2024
Conoscenze e abilità da conseguire
To know the fundamentals of applied biochemistry and microbiology and technology necessary for the development, management and optimization of existing and new bioprocesses for the sustainable chemical and biobased industry (biorefineries), the valorization of their waste and effluents.
Contenuti
Nutritional requirements of micro-organisms and microbial metabolisms of interest for industrial and environmental bioprocesses.
Aerobic chemoorganotrophic respiration (glycolysis, Krebs cycle and oxidative phosphorylation). Aerobic chemolithotrophic respirations (hydrogenotrophic respiration, nitrification, S° or S= -oxidation, Fe(II) oxidation). Examples and main features of industrial and environmentally relevant microorganisms respiring aerobically.
Anaerobic respirations (nitrate-reduction, Fe(III)-reduction, sulphate-reduction, HCO3— reduction (methanogenesis and acetogenesis)). Main features of major industrially and environmentally relevant microorganisms using anaerobic respiration routes. Fermentation of carbohydrates and proteins, fermentation pathways of major relevance for industrial and environmental processes and main features of microorganisms responsible for them. Anaerobic digestion of organic matter and biowaste and its industrial and environmental relevance.
Operational steps and examples of industrial biotechnological processes in the food/feed, pharma and chemical industry
Feed-stocks for white biotechnology processing (agricultural and forest biomass, and agro-food industry by-products, effluents, waste and surplus) and their pre-treatment. Mixed cultures, co-cultures and pure (axenic) cultures, procedures for their characterization and conservation. Selection and improvement of the microbial strain. Selection and preparation of the cultivation medium. Preparation of the inoculum. Selection of the bioreactor. Sterilization of the bioreactor and the cultivation medium. Conduction mode of the process (batch, fed-batch and continuous). Steps in the recovery and purification of the product.
Biochemical, microbiological and technological aspects of industrial biotechnological processes for the production of yeast, microbial proteins, amino acids (L-glutamic acid, L-lysine), vitamins (B12, C), organic acids (citric, lactic), antibiotics (penicillins), biofuels, monomers or monomer precursors (lactic acid, butandiol, ethanol) for biobased polymers, microbial polymers (polyhydroxyalkanoates).
Adaptation and exploitation of microorganisms in environmental biotechnological processes for the bioremediation of contaminated sites
Occurrence and distribution of different microbes and microbial processes in the major aquatic and terrestrial natural habitats.
Major organic and inorganic xenobiotic pollutants entering the environment and microorganisms mainly involved in their biotransformation and detoxification. Expansion of catabolic pathways and main biodegradation pathways for aliphatic and aromatic hydrocarbons and chlorinated hydrocarbons in aerobic and anaerobic polluted environments.
Main techniques for the in-situ and ex-situ bioremediation of contaminated soils and aquifers: microbiological and technological aspects of bioventing, pump&treat, biobariers and reactive permeable barriers, landfarming, biopiles and treatment in bioreactors.
Testi/Bibliografia
Madigan M.T. Martinko J.M., Parker J. (2002) Brock: Biology of Microorganisms.10th Edition, Prentice Hall International, Inc . New Jersey, USA
Ratledge C., Kristiansen B. (2006) Basic Biotechnology, III Edition, Cambridge University Press, London
Glazer A.N., Nikaido H. (1997) Microbial Biotechnology- Fundamentals of Applied Microbiology. 2nd Edition. WH Freeman and Company, New York.
Ulber R., Sell D. (2007) White Biotechnology, Vol. 105 of the series Advances in Biochemical Engineering / Biotechnology, Springer, EU and USA.
Rittmann B.E., McCarty P.L. (2001) Environmental Biotechnology: principles and applications. McGraw-Hill Higher education, New York, USAMetodi didattici
Class lectures
Modalità di verifica e valutazione dell'apprendimento
Achievements will be assessed by the means of a final oral exam, through which the knowledge level acquired by the student on the topics covered in the class, his/her ability to present them clearly and with command of language and to discuss them critically will be evaluated.
Higher grades will be awarded to students who demonstrate an organic understanding of the subject, a high ability for critical application, and a clear and concise presentation of the contents.
To obtain a passing grade, students are required to at least demonstrate a knowledge of the key concepts of the subject, some ability for critical application, and a comprehensible use of technical language.
A failing grade will be awarded if the student shows knowledge gaps in key-concepts of the subject, inappropriate use of language, and/or logic failures in the analysis of the subject.Orario di ricevimento
Consulta il sito web di Giulio Zanaroli
SDGs


L'insegnamento contribuisce al perseguimento degli Obiettivi di Sviluppo Sostenibile dell'Agenda 2030 dell'ONU.