Anno Accademico 2023/2024

  • Docente: Francesco Alviano
  • Crediti formativi: 4
  • SSD: BIO/17
  • Lingua di insegnamento: Inglese
  • Moduli: Francesco Alviano (Modulo 1) Pasquale Marrazzo (Modulo 2)
  • Modalità didattica: Convenzionale - Lezioni in presenza (Modulo 1) Convenzionale - Lezioni in presenza (Modulo 2)
  • Campus: Bologna
  • Corso: Laurea Magistrale in Medical Biotechnology (cod. 9081)

Conoscenze e abilità da conseguire

Identify and discuss stem cell features, molecular mechanisms of differentiation and possible applications related to regenerative medicine. Recognise the characteristics of embryonic stem cells and those of the adult stem cells. Describe the methods for stem cell isolation to obtain progenitor cells from extraembryonic tissues.​


Frontal lessons (2 UC)

Mesenchymal Stem Cells (MSCs): historical background, isolation, immunophenotypic characterization, differentiation ability, trophic and immunomodulatory features.

Alternative source and clinical perspectives of MSCs.

Embryonic Stem Cells (ESCs) and induced Pluripotent Stem Cells (iPSCs).

Resident angiogenic mesenchymal stem cells from heart-beating multiorgan donors

Stem cells for the regeneration of chondrogenic and osteogenic.

The regeneration of the skin. The stem cell niches of epithelial stem cells.

3D Organoid System: 3D Scaffold application in cell culture and organoid models: tissue engineering approach for disease modelling and regenerative medicine approaches.

Cell Culture Laboratory for studying stem cells. The risks of contamination: cryopreservation.

The safety devices in the cell culture laboratories.

Lab work experience (2 UC)

1- Demonstration of human term placenta. Human mesenchymal cell isolation from the Wharton's jelly of the umbilical cord;

2 - Isolation of epithelial cells from the amniotic membrane;

3 - Isolation of mononuclear cells from blood and preparation of a co-culture to evaluate the immunomodulatory action of the epithelial cells isolated from amniotic membrane;

4 - Evaluation of the cycle phases of mononuclear cells maintained in co-cultured with epithelial cells of the amniotic membrane;

5 - Evaluation of perinatal cell differentiation potential by attempting an angiogenic assay based on a 3D semi-solid culture system.

Discussion of data and comparison with those reported by scientific literature.


Recommended Textbook: G.P. Bagnara, Stem Cells, Ed. Esculapio.

Recommended scientific papers given during the lessons.

Metodi didattici

Frontal lectures and Laboratory work experience in Cell Culture Lab.


As concerns the types of activities and teaching methods adopted, the attendance of this training activity requires the performance of all students of Modules 1 and 2 in e-learning mode and the participation in Module 3 of specific training on safety and health in the places of study. Information on dates and methods of attendance of Module 3 can be consulted in the specific section of the degree program website.

Modalità di verifica e valutazione dell'apprendimento

The final test focuses on the achievement of learning objectives about:

- stem cell basics and classification

- stem cell applications, cell therapy and tissue engineering

- stem cell research and techniques

Oral examination on two topics among a list of topics covered during the course.

The final mark of the "Stem Cell Therapy" course will be mediated with the marks reported in the “Human Embryology course and with the “Molecular Basis of Haematological Disorders” course.

The online registration to the examination is mandatory.

Strumenti a supporto della didattica

PowerPoint presentations that will be available online as downloadable content.

Orario di ricevimento

Consulta il sito web di Francesco Alviano

Consulta il sito web di Pasquale Marrazzo


Salute e benessere Istruzione di qualità

L'insegnamento contribuisce al perseguimento degli Obiettivi di Sviluppo Sostenibile dell'Agenda 2030 dell'ONU.