85299 - EPIGENOMICS

Conoscenze e abilità da conseguire

Al termine del corso, lo studente ha familiarizzato con i meccanismi alla base dei fenomeni epigenetici coinvolti nella regolazione genica e nella organizzazione spaziale dei genomi. In particolare, lo studente comprende l'utilizzo dei saggi molecolari che generano dati massivi riguardo al set completo delle modificazioni e delle molecole epigenetiche e del loro impatto sull'espressione genica, stabilità genomica, differenziamento cellulare e oncogenesi.

Contenuti

Lecture 1: Historical notes about the birth of Epigenetics as a new scientific discipline. Basic concepts and analysis of phenomena that apparently do not follow mendelian laws.

Lecture 2: Epigenome: Expressivity of the genome independent from DNA sequence. Analysing genetic inheritance beyond DNA sequence.

Lecture 3: Defining multiple transcriptomes from the same informational DNA sequence. Understanding how transcription works: Basal transcription:

Lecture 4: Defining multiple transcriptomes from the same informational DNA sequence. Understanding how transcription works:Regulated transcription:

Lecture 5: Genome wide analyses of the transcriptome from sequence of bulk biological material

Lecture 6:Genome wide analyses of the transcriptome from single cell sequencing

Lecture 7: Defining multiple transcriptomes from the same informational DNA sequence. Understanding how transcription works: Histones and transcription.

Lecture 8: Histones as targets of DNA re-modeling complexes.

Lecture 9: Histones as targets of multiple post-translational modifications.

Lecture 10: Modifiers, Readers and Erasers of epigenetic marks.

Lecture 11: Genome wide analyses of chromatin architecture: ChIP and ChIP-seq

Lecture 12:Genome wide analyses of chromatin architecture: 3C, 5C, iC.

Lecture 13: The DNA methylation problem. CpGs or not CpGs. CpG islands.

Lecture 14: De novo or maintenance of DNA methylation. DNMTs and their function during development.

Lecture 15: Analyses of the DNA methylation status: use of methylation sensitive REs; MS-PCR bisulfite sequencing, COBRA.

Lecture 16: Genome wide DNA methylation: Methyl-DIP; definition of the methylome by NGS.

Lecture 17: Histone modifications meet DNA methylation

Lecture 18: Molecular epigenetic memory.

Lecture 19: Case study 1: What is known/posing questions

Lecture 20: Case study 1: Brain storming/ problem solving

Lecture 21: Case study 2: What is known/posing questions

Lecture 22: Case study 2: Brain storming/ problem solving

Lecture 21: Case study 3: What is known/posing questions

Lecture 22: Case study 3: Brain storming/ problem solving

 

 

 

Testi/Bibliografia

EPIGENETICS II edition CSHL edited by D. Allis

ISBN 978-1-936113-59-0

papers from scientific literature

Metodi didattici

Lectures, group discussion, brain storming, flipped classroom

Modalità di verifica e valutazione dell'apprendimento

Brief written dissertation or ppt presentation describing analyses and possible solutions of relatively simple still unsolved questions that came out during brain storming.  

Strumenti a supporto della didattica

white board, video-projection, origami class, pc.

Orario di ricevimento

Consulta il sito web di Giorgio Milazzo