93272 - ADVANCED IMMUNOLOGY

Conoscenze e abilità da conseguire

Alla fine delle lezioni, lo studente dovrebbe conoscere i meccanismi cellulari e molecolari che regolano il funzionamento del sistema immunitario, nonché le attuali e moderne possibilità di dispiegare attività immunitarie a fini terapeutici. In particolare, lo studente conoscerà: i) le strategie genetiche che consentono la diversificazione delle risposte immunitarie agli antigeni; ii) la generazione e l'uso di anticorpi monoclonali; iii) strategie di immunosoppressione; iv) generazione di recettori dell'antigene chimerico.

Contenuti

Course contents

• Receptors of the natural Immunity: TLR, NLR and inflammasomes, cGAS-Sting, RLR, etc. Connection with inflammation. PAMPs and DAMPs. Regional Immunity. Interactions between immune cells and gut microbiota.
• Organization of genetic loci for the receptors of NK cells. Memory of the innate immunity: trained immunity.
• Organization of genetic loci for antibodies and TCR. Generation of receptor repertoire: mechanisms of combinatorial differentiation (somatic recombination), and junctional differentiation.
• Molecular mechanisms of the isotypic shift; affinity increase and somatic hypermutation.
• Synthesis of monoclonal antibodies. Nomenclature CD. Immunophenotypization with flow cytometry and other methods. Cell sorting and single-cell analysis.
• Applications of monoclonal antibodies in biomedical research, diagnostics and clinic practice.
• Mechanisms of gene regulation: DNA methylation. Experimental data of methylation changes with age.
• Organization of MHC genetic locus. Polymorphism of MHC genes. Genes for MHC I, II and III. HLA genotyping. Antigen presentation on MHC class I or II molecules. MHC in diagnostics: the tetramers. Macroautophagy and chaperon-mediated autophagy: importance for the immune responses.
• Tolerance breakdown. Autoimmunity. Immunopathology: classification of hypersensitivities. Allergies: features and pathogeneic mechanisms; Type II, III and IV hypersensitivities.
• Congenital and acquired immunodeficiencies. SCID and other immunodeficiencies of lymphocyte maturation. Innate immunity Immunodeficiencies. Acquired Immunodeficiencies due to immunosuppression, chemotherapy, malnutrition, viral infections.
• Cancer Immunology: immunological features of tumors. Cancer Antigens. Cancer Immunotherapy: monoclonal Abs. Anti-PD-1 and PD-L1; CAR-T cells: generation and use.
• Aging of the immune system: immunosenescence and inflammaging. Cell senescence and SASP: the role in human aging and age-related diseases. Inflammaging and the special case of Covid-19.

Testi/Bibliografia

Abbas, Lichtman, Pillai: Cellular and molecular immunology, 2022 (tenth edition), Elsevier.

Kenneth Murphy & Casey Weaver: Janeway's Immunobiology, 2022 (tenth Edition), Garland Science.

Metodi didattici

Frontal lessons with ppt slides unless otherwise stated by current provisions.

Modalità di verifica e valutazione dell'apprendimento

Oral examination in presence. Online exams with Microsoft Teams platform in oral form are allowed only upon official clearance by the Study Course Secretary. In this case, students must register as usual in Almaesami, and they will receive an e-mail with the link and timetable of the exam session.

Strumenti a supporto della didattica

Ppt files available at VIRTUALE platform for UNIBO students.

Orario di ricevimento

Consulta il sito web di Stefano Salvioli