90920 - STEM CELLS AND 3D ORGANOID SYSTEMS

Anno Accademico 2021/2022

Conoscenze e abilità da conseguire

Alla fine del corso lo studente conosce: - i percorsi molecolari che controllano il grado di staminalità e il potenziale di differenziazione delle cellule staminali; - gli elementi chiave riguardanti le innovazioni e lo sviluppo dei sistemi di cultura 3D per la scoperta di farmaci e il loro uso per la modellizzazione della malattia; - i metodi per la preparazione in vitro di modelli cellulari utili per la definizione di colture sferoidali 3D.

Contenuti

Frontal lessons (2 UC)

Pluripotent stem cells. Embryonic stem cells: phenotypic characterization and therapeutic potential.

Somatic nuclear transfer for the generation of pluripotent cells. Reprogrammng strategies for generation of induced pluripotetn stem cells (iPSCs).

Adult stem cells: phenotypic characterization, multipotential ability and comparison with Pluripotent Stem Cells.

Extraembryonic Tissues as sources of Perinatal Stem Cells: phenotypic characterization and biologial features.

3D Scaffold application in cell culture and organoid models: tissue engineering approach for disease modelling and regenerative medicine approaches.

3D organoid development and growh: general overview of the background and current methods for the generation and characterization of organoids

Application of human derived organoids: cerebral, liver, skin and intestinal model of organoids.

 

Lab work experience (1 UC)

Introduction to the lab work of the stem cell course; macroscopic description of the placenta and extraembryonic tissues; laboratory experience: isolation of human mesenchymal cells derived from fetal membranes.

Laboratory experience: isolation of human epithelial cells derived from amniotic membrane and preparation of 3D cellular models for 3D culture system.

Laboratory experience: Morphological evaluation of the cell cultures obtained and discussion of research findings.

 

Testi/Bibliografia

Recommended Textbook: G.P. Bagnara, Stem Cells, Ed. Esculapio.

Recommended scientific papers given during the lessons.

Metodi didattici

Frontal lectures and Laboratory work experience in Cell Culture Lab.

Modalità di verifica e valutazione dell'apprendimento

The final test focuses on the achievement of learning objectives about:

- stem cell basics and classification

- stem cell applications, cell therapy and tissue engineering

- 3D organoid l research and techniques

Oral examination on two topics among a list of topics covered during the course. 

The online registration to the examination is mandatory.

Strumenti a supporto della didattica

PowerPoint presentations will be available online as downloadable content.

Orario di ricevimento

Consulta il sito web di Francesco Alviano