90918 - ELEMENTS OF GENETICS

Scheda insegnamento

Anno Accademico 2021/2022

Conoscenze e abilità da conseguire

Al termine del corso, lo studente possiede una conoscenza profonda circa i principi fondamentali dell'ereditarietà. Ha familiarità con la natura, trasmissione, espressione variabilità e manipolazione del materiale ereditario ed è in grado di comprendere, interpretare e valutare criticamente in modo rigoroso i dati sperimentali genetici.

Contenuti

  • Introduction to genetics
  • Mendelian Genetics
    • Gregor Mendel and basic principles of heredity; monohybrid crosses (dominance and segregation); dihybrid crosses (independent assortment)
    • Predicting the outcome of genetic crosses; probability and Chi-Square test
  • Cell division, mitosis and meiosis, chromosomal basis of inheritance
    • Sexual reproduction, mitosis and meiosis.Chromosome theory of inheritance
    • Sex determination and sex-linked inheritance
    • Dosage compensation in mammals
  • Human pedigree analysis
  • Extensions and modifications of basic mendelian principles
    • Allelic variation and gene function; why some alleles are dominant and other recessive; types of dominance, reduced penetrance, lethal alleles.
    • Genetic heterogeneity, gene interactions and epistasis.
  • Linkage, recombination, gene mapping in Eukaryotes
    • Linked genes and crossing over; Constructing genetic maps with recombination frequencies
    • Linkage analysis in human; DNA polymorphisms as genetic markers; the lod-score method
  • Overview of genetics of bacterial and viral genetic systems
  • Genetic variation, DNA repair and recombination
    • Genetic variability; mutation and polymorphisms; types of genetic variants; molecular basis of mutations; point mutations and their consequences; mutagenesis
    • DNA repair mechanisms; DNA recombination mechanisms
    • Variation in chromosome number and structure; mechanisms of structural variation

Testi/Bibliografia

Leland Hartwell, Michael L. Goldberg, Janice Fischer and Leroy Hood

6th edition

McGrawHill Education

Other textbooks:

Introduction to Genetic Analysis, by Griffiths, Wessler, Carroll, and Doebley.

Principles of Genetics , by D. Peter Snustad, Michael J. Simmons

Genetics. Analysis & Principles, by Robert J.Brooker

Concepts of Genetics, by Klug, Cummings, Spencer

Metodi didattici

Lectures with powerpoint presentations; in class problem solving and exercise

Modalità di verifica e valutazione dell'apprendimento

Final written and oral exam. Written will include multiple choice questions, questions with open answers and problem solving

Strumenti a supporto della didattica

Course materials (ppt presentations, problems and solutions, suggested readings) will be made available to students

Orario di ricevimento

Consulta il sito web di Giorgio Milazzo