Abstract
Ascorbate (ASC) is a powerful antioxidant in plants. Cytochrome b561 (cyb561) connect the redox states of cytoplasmic and extracytoplasmic ASC pools in plant cells. Our preliminar results show that knock out mutants of two members of the cyb561 family have symptoms that recall those of mutants of ASC metabolism, including the different timing of flower induction. A major goal of the TASCO project is to understand to which extent cyb561s may control ASC homeostasis, thereby explaining how flowering induction may be modulated by ASC-mediated trans-membrane electron transport in mesophyll cells. By means of electrophysiological techniques we expect to determine the kinetic parameters of different Cyb561 isoforms and mutants. These results will be interdisciplinary connected to the phenotypic and transcriptomic analysis of knock out mutants. All together, the expected results will shed new light on the molecular basis of flowering in Arabidopsis.
Dettagli del progetto
Responsabile scientifico: Paolo Bernardo Trost
Strutture Unibo coinvolte:
Dipartimento di Farmacia e Biotecnologie
Coordinatore:
ALMA MATER STUDIORUM - Università di Bologna(Italy)
Contributo totale di progetto: Euro (EUR) 190.013,00
Contributo totale Unibo: Euro (EUR) 97.415,00
Durata del progetto in mesi: 24
Data di inizio
05/10/2023
Data di fine:
28/02/2026
