99474 - Prokaryotic Genomes

Learning outcomes

By the end of the module the student has knowledge of the main approaches for genomic data analysis and annotation in prokaryotes, with an emphasis on the use of next generation sequencing for the functional and structural analysis of genomes. In particular the student is able to: understand the structure of genetic variability and its phenotypic effects, browse prokaryotic genomes, apply methods for genomic data analysis, and correctly interpret and plan genomic studies in bacteria.

Course contents

Readings/Bibliography

Required Material for Exam Preparation

* Lecture slides and teaching materials provided by the instructor and made available through the Virtuale platform.

* Scientific articles and review papers assigned during the course.

Recommended Reading

* Snyder L.A.S. (2020). *Bacterial Genetics and Genomics*. CRC Press, Taylor & Francis Group.

* Brown T.A. (2023). *Genomes 5*. CRC Press, Taylor & Francis Group.

Additional scientific papers and review articles will be recommended throughout the course.

 

Teaching methods

The course is delivered through:

* lectures supported by multimedia presentations;

* discussion of applied examples and case studies;

* hands-on bioinformatics practical sessions conducted in the computer laboratory as part of Module 2;

* guided activities aimed at the use of genomic databases and tools for prokaryotic genome analysis.

Teaching activities are designed to provide students with both theoretical knowledge and practical skills required for the analysis, interpretation, and comparative study of prokaryotic genomes.

Due to the nature of the activities and teaching methods adopted, participation in the computer laboratory sessions requires prior completion of Modules 1 and 2 of the University's online health and safety training programme.

Students have the right to refuse the registration of a positive grade once, in accordance with the University Teaching Regulations (Art. 16, paragraph 5).

Students with Specific Learning Disorders (SLD) or temporary/permanent disabilities are encouraged to contact the relevant University Office (https://site.unibo.it/studenti-con-disabilita-e-dsa/it) well in advance. The Office will propose any necessary accommodations, which must be submitted to the instructor for approval at least 15 days before the examination date. The instructor will evaluate the appropriateness of the requested accommodations in relation to the learning objectives of the course.

Students with officially recognized “working student” status should refer to the dedicated University webpage (https://www.unibo.it/it/studiare/guida-alla-scelta-del-corso/conciliare-studio-e-lavoro) for information on eligibility requirements and available support measures.

 

Assessment methods

The final examination is designed to assess:

* knowledge of the principles of prokaryotic genetics and genomics;

* understanding of the mechanisms underlying genome evolution and plasticity;

* ability to interpret genomic and transcriptomic data;

* ability to correctly use the scientific terminology of the field.

The final grade is based on the following assessments.

Oral Examination (Module 1)

The oral examination evaluates:

* understanding of the topics covered during the course;

* ability to connect different topics within the syllabus;

* critical reasoning skills;

* mastery of scientific terminology.

Written Examination (Module 2) 

Option A (available exclusively during the 1st exam session)

The written examination includes:

* multiple-choice questions;

* short-answer questions;

* open-ended theoretical and applied questions.

Option B (available during all the other exam sessions)

The written examination consists of **15 short open-ended questions** covering the theoretical and applied topics addressed in Module 2.

Answers should be concise, with a maximum length of **10 lines per question**. 

The written examination is designed to assess both theoretical knowledge and the ability to interpret problems and datasets related to prokaryotic genomics.

Assessment Criteria

Evaluation will take into account:

* accuracy and completeness of the acquired knowledge;

* ability to apply theoretical concepts to problems in microbial genomics;

* ability to interpret experimental data and results;

* appropriate use of scientific terminology;

* clarity of presentation and ability to establish connections among the topics covered.

Indicatively:

* 18–21: knowledge of fundamental concepts and basic understanding of the topics covered;

* 22–25: good knowledge of the subject matter and ability to apply acquired concepts;

* 26–29: thorough knowledge, ability to integrate different topics, and good critical analysis skills;

* 30–30 cum laude: comprehensive mastery of the subject, excellent critical thinking and integrative skills, and rigorous use of scientific terminology.

During examinations, the use of textbooks, notes, electronic devices, or any other supporting materials is not permitted unless otherwise specified by the instructor. 

The use of AI is prohibited in all assessments. Any use of AI constitutes a breach of academic integrity.

Teaching tools

The course makes use of:

* PowerPoint presentations made available through the Virtuale platform;

* selected scientific articles and review papers;

* supplementary teaching materials provided by the instructor;

* software and genomic databases used during the bioinformatics practical sessions;

* open-source videos supporting specific course topics, where appropriate.

Teaching materials are selected to promote independent learning and ensure accessibility of course content for all students.

Links to further information

https://site.unibo.it/molecular-environmental-microbiology-lab/it/teaching

Office hours

See the website of Martina Cappelletti

See the website of Marco Rinaldo Oggioni