B3276 - Structures and functions of RNAs and their modulation by small molecules

Learning outcomes

Upon completion of the course, the student has a solid theoretical foundation for understanding the relationships between RNA structure and its regulatory and catalytic functions with possible therapeutic activities.

Course contents

RNA polymerase and classes of RNA. Structure of RNA polymerase II. Organization of class I, II and III promoters. General transcription factors and their functions. Structure of TBP in complex with DNA. Regulation of transcription. Upstream and inducible transcription factors. Enhancers. Maturation of transcripts. Secondary and tertiary structural motifs of DNA and RNA, examples of structured nucleic acids (riboswitches, ribozymes, long regulatory RNAs, ribonucleoprotein complexes in which catalytic activity is carried out by RNA subunits), recognition of metabolites and drugs by nucleic acids, use of small molecules for regulation of nucleic acid functions. The student will also learn the main experimental technologies used for the production, purification and structural characterization of RNA in vitro.

Readings/Bibliography

Molecular Biology - Weaver - McGraw Hill


Scientific literature provided in lecture in pdf format.

Teaching methods

Classroom lectures.

Assessment methods

Evaluation of scientific seminar on specific assigned topic.

Teaching tools

Students with Specific Learning Disorders (SLD) or temporary/permanent disabilities are advised to contact the appropriate University Office in good time (https://site.unibo.it/studenti-con-disabilita-e-dsa/en). The office will be responsible for proposing any necessary accommodations to the students concerned, which must in any case be submitted to the course instructor for approval at least 15 days in advance. The instructor will evaluate their appropriateness in relation to the learning objectives of the course.

Office hours

See the website of Jessica Marinello