69137 - Obstetrics and gynaecology Laboratory

Academic Year 2014/2015

  • Docente: Antonio Farina
  • Credits: 2
  • SSD: MED/40
  • Language: Italian
  • Teaching Mode: In-person learning (entirely or partially)
  • Campus: Bologna
  • Corso: Second cycle degree programme (LM) in Medical Biotechnology (cod. 8508)

Learning outcomes


Course contents

Non invasive prenatal  diagnosis
1. Combined test
     Risk calcualation of Down Syndrome and other aneuploidies by using  nuchal transulcency,  PAPP-A and  fbhCG
     MoM, Likelihood Ratio, Detection Rate, risk in positive
     Performance indicators of  Down S. screening (Sensitivity or Detection Rate, False Positive Rate, Risk in Positive)

2.  Fetal DNA circulating in maternal  plasma for  the prenatal diagnosis of  Down Syndorme and other  aneuploidies
     Methods of sequences analysis of fetal DNA (Massevly Parallel Shotgun Sequencing, Target Sequencing Approach, Single Nucleotide Polymorphisms)
     Performanceof  fetal DNA screening
     Problems f false positive and false negative in fetal DNA screening: Mosaicisms, Vaninshing Twin, Z-score and low  fetal fraction frazion

3. Fetal RhD diagnosis from matermal blood
    Description of  RhD system  and alloimmunizzation syndrome
    Cenni di clinica della sindrome da alloimmunizzazione e follow-up della donna isoimmunizzata
    Metodi e strategie di amplificazione (Real Time PCR Multiplex)
    Il problema degli pseudogeni nella amplificazione
    I falsi positivi e i falsi negativi

Diagnosi prenatale invasiva

1 .  Villi Coriali per la determinazione del cariotipo e delle malattie Mendeliane
       Indicazioni, vantaggi, svantaggi, gestione dei mosaicismi
2.    Amniocentesi per la determinazione del cariotipo
       Indicazioni, vantaggi, svantaggi,        
       QF-PCR indicazioni vantaggi svantaggi
       FISH interfasica e metafasica, indicazioni vantaggi svantaggi
       GCH Array indicazioni svantaggi, vantaggi


Readings/Bibliography


Assessment methods


Teaching tools


Office hours

See the website of Antonio Farina