- Docente: Michela Rugolo
- Credits: 3
- SSD: BIO/10
- Language: Italian
- Teaching Mode: In-person learning (entirely or partially)
- Campus: Bologna
- Corso: Second cycle degree programme (LS) in Industrial Molecular Biotechnologies (cod. 0444)
Learning outcomes
Detailed knowledge of some important signalling mechanisms operating in animal cells and description of the experimental strategies utilized to achieve this knowledge.
Course contents
Protein kinases and phosphatases: classification and functionl domains of PKA and PKC. PPases: molecular organization . AKAP: specificity of kinases and PPases.
Rhodopsin and the family of seven helical receptors: 3D structure and functional domains. Signalling strategies of seven helical receptors: dependent and indepedent from G proteins. receptor inactivation: the GRK family. Arrestins as signalling molecular switchs
PLC and adelylate cyclase as models for effector systems.
Calcium as universal messenger: transpoters and pumps in cellular membranes. IP3 and ryanodine receptors. Other intracellular calcium mobilizators: cADPR and NAADP.. Temporal and spatial aspects of calcium signalling. Capacitative calcium entry. Calcium and mitochondria: studies with recombinant aequorin.
Cyclic AMP: in vivo measurements with FRET: spatial and temporal aspect of signalling
Mitochondria: Mitochondrial ultrastructure; determination of the morphological and functional heterogenicity of the mitochondrial network in vivo by means of fluorescent dyes and proteins. Contact sites between the mitochondrial membranes: VDAC and the energetic function. Proteins involved in the regulation of mitochondrial fusion and fission and apoptosis. The mitochondrial DNA.
Readings/Bibliography
Scientific articles
Teaching methods
Lectures
Assessment methods
Oral examination
Teaching tools
Powerpoint presentations and papers given by the teacher during the course
Office hours
See the website of Michela Rugolo