Abstract
Ascorbate (ASC) is a powerful antioxidant in plants. Cytochrome b561 (cyb561) connect the redox states of cytoplasmic and extracytoplasmic ASC pools in plant cells. Our preliminar results show that knock out mutants of two members of the cyb561 family have symptoms that recall those of mutants of ASC metabolism, including the different timing of flower induction. A major goal of the TASCO project is to understand to which extent cyb561s may control ASC homeostasis, thereby explaining how flowering induction may be modulated by ASC-mediated trans-membrane electron transport in mesophyll cells. By means of electrophysiological techniques we expect to determine the kinetic parameters of different Cyb561 isoforms and mutants. These results will be interdisciplinary connected to the phenotypic and transcriptomic analysis of knock out mutants. All together, the expected results will shed new light on the molecular basis of flowering in Arabidopsis.
Project details
Unibo Team Leader: Paolo Bernardo Trost
Unibo involved Department/s:
Dipartimento di Farmacia e Biotecnologie
Coordinator:
ALMA MATER STUDIORUM - Università di Bologna(Italy)
Total Eu Contribution: Euro (EUR) 190.013,00
Total Unibo Contribution: Euro (EUR) 97.415,00
Project Duration in months: 24
Start Date:
05/10/2023
End Date:
28/02/2026
