Abstract
The objective of Nymphe is to develop bioremediation/revitalization strategies based on the assembly of systems of available and new biologics (enzymes, microorganisms, bivalves and earthworms, plants and their holobiont) developed and applied on matrices from different EU contaminated sites: groundwater, sediments (hyporheic zone), wastewater as well as industrial, and agricultural soils. The assembly of systems is guided using predictive microbial interaction models in the microbiome of the actual site matrix to be decontaminated. The best systems of biologics are reintroduced at the contaminated sites (4 plus 1 site for confined tests using genomically edited biologics). Nymphe target at least 90% removal of the pollutants occurring at the sites, e.g. plastics and pesticides in the agricultural soil, and chlorinated solvents/total petroleum hydrocarbon in groundwater and sediments of the industrial site. Besides, biologics and systems benefit from the development of platform technologies to: assemble stable consortia (adhesin +complementary auxotrophies), accelerate the evolution of the microorganisms, increase mobility of degradative plasmids, genomically edit bacteria to produce evolved enzymes, produce enzyme systems with recycling of cofactors, control the spatial distribution of biodegraders (3D-printing), protect them in harsh environments, facilitate redox processes by electromicrobiology, design self-sustaining redox biocatalysts. The bioremediation/revitalization strategies undergo a full assessment: ecological and environmental risk analyses (chemical, ecotoxicological and supportive analyses e.g., function and biodiversity of the microbiomes), technology risk analysis, life cycle assessment, life cycle cost, cost-efficiency analysis, biosafety & regulatory constraints analysis, and societal analysis to be finally benchmarked with current solutions. The ecological status of the bioremediated sites shall be closer to Natura 2000 specifications
Project details
Unibo Team Leader: Giulio Zanaroli
Unibo involved Department/s:
Dipartimento di Ingegneria Civile, Chimica, Ambientale e dei Materiali
Dipartimento di Farmacia e Biotecnologie
Coordinator:
ALMA MATER STUDIORUM - Università di Bologna(Italy)
Other Participants:
Universitat De Girona
(Spain)
Total Eu Contribution: Euro (EUR) 5.499.973,50
Project Duration in months: 48
Start Date:
01/01/2023
End Date:
31/12/2026
This project has received funding from the European Union’s Horizon Europe research and innovation programme under grant agreement No 101060625