90947 - Pediatrics

Learning outcomes

Mod.1: screening of translocations with prognostic significance in pediatric acute leukemias .In vitro reproduction of translocations of pediatric oncohematological interest through editing with CRISPR / Cas9. Methods used: electroporation, extraction of nucleic acids, amplification of the fusions with PCR, running on agarose gel and analysis of the Mod.2: basic knowledge of the physiological and pathological mechanisms underlying the main diseases of preterm and term neonates and of the related diagnostic and therapeutic techniques. In particular the following conditions will be addressed: preterm birth – respiratory distress – bronchopulmonary dysplasia – necrotizing enterocolitis – retinopathy of prematurity – neurological diseases of preterm infants – hypoxic-ischemic encephalopathy. The course will also focus on the techniques used for neonatal haemodynamic monitoring (including NIRS, electrical cardiometry, ultrasounds) and on the role of microbiome in neonatal diseases. Mod.3: To acheive a basic knowledge of physiological and pathological processes regulating the growth and the development of children in particular in regard to the following aspects: - Ages of life - Development, growth and related disorders - Microbiota in pediatrics- Pediatric leukemia and biological background- Pediatric solid tumors and biological background - Genetic background of pediatric tumors and cancer predisposition - Chromosomal aberrations and pediatric syndromes- Vaccinations

Readings/Bibliography

Nelson Textbook of Pediatrics

Slides

Teaching methods

Frontal Lessons

Supplementary materials will be available in pdf and PPT

Clinical case reports will be discussed

Mentimeter quiz will be used to test, engage and entertain the students during the course

Assessment methods

The purpose of the oral exam is to verify the student's ability to apply his or her knowledge and to make the necessary logical-deductive connections. Gradation of the final grade: Preparation on a very limited number of topics covered in the course and analytical skills that emerge only with the help of the teacher, expressed in an overall correct language → 18-19; Preparation on a limited number of topics covered in the course and ability to autonomous analysis only on purely executive matters, expression in correct language → 20-24; Preparation on a large number of topics covered in the course, ability to make independent choices of critical analysis, mastery of specific terminology → 25-29; Basically exhaustive preparation on the topics covered in the course, ability to make autonomous choices of critical analysis and connection, full mastery of specific terminology and ability to argue and self-reflection → 30-30L.

Teaching tools

ower point Lessons

Mentimeter quiz

Other Supplementary materials (Articles, Videos etc)

Office hours

See the website of Riccardo Masetti

See the website of Francesca Conti