14041 - Biochemistry Lab-based Course

Learning outcomes

At the end of the module the student acquires methodological and applicative elements useful for integrating the knowledge gained in the courses of Chemistry and Biochemistry.

Course contents

The course will be divided into a part of frontal teaching in which the following topics will be addressed:

Techniques for studying the structure of proteins

XRD, NMR, cryo-EM

Main analytical techniques used in biochemistry

Spectrophotometry

Fluorescence

Purification and separation techniques

Ultracentrifugation

Electrophoresis

Western Blotting

Three laboratory experiments will be carried out:

1. computer laboratory on the structure of proteins
2. chemistry laboratory on redox and acid-base properties
3. biochemical laboratory on enzymatic kinetics and protein quantification.

Readings/Bibliography

PowerPoint presentations integrated by personal notes can provide useful support for the study of the subject.

Teaching methods

Frontal and online lessons based on Powerpoint presentations. Laboratory exercises and subsequent data processing.

In consideration of the types of activities and teaching methods adopted, the attendance of this training activity requires all students of Modules 1 and 2 to carry out e-learning: [https://www.unibo.it/en/services-and-opportunities/health-and-assistance/health-and-safety/online-course-on-health-and-safety-in-study-and-internship-areas] and participation in Module 3 of specific training on safety and health in study places. Information on dates and methods of attendance of Module 3 can be consulted in the specific section of the degree program website.

Assessment methods

The laboratory course program is not included in the exam of the I.C. of Biochemistry.

Students should write a brief report containing the analytical data on enzymatic kinetics and proteins' quantification.

Teaching tools

Tutte le lezioni saranno supportate da presentazioni Power-Point, rese disponibili prima delle lezioni.

Office hours

See the website of Michele Di Foggia