Abstract
The ESILARANTE project aims to develop an innovative portable biosensing platform for the detection of cannabinoid in capillary blood samples, based on laccase biosensors fabricated via Electrospray Deposition (ESD), a green and cost-effective enzyme immobilization technique. The project combines enzyme purification, physico-chemical characterization and advanced electrochemical testing to identify the factors enabling high sensor stability and reusability. Particular attention is given to the analytical performance, with quantitative evaluation of enzymatic activity and validation against fully validated LC-MS/MS protocols. Analytical techniques also investigate enzyme integrity before and after deposition, helping to optimize the system. The expected result is a robust, selective, and miniaturized biosensor for rapid, on-site applications in forensic and roadside settings, representing a sustainable and scalable alternative to conventional laboratory-based cannabinoid testing.
Project details
Unibo Team Leader: Michele Protti
Unibo involved Department/s:
Dipartimento di Farmacia e Biotecnologie
Coordinator:
CNR - Consiglio Nazionale delle Ricerche(Italy)
Total Unibo Contribution: Euro (EUR) 79.000,00
Project Duration in months: 24
Start Date:
28/09/2023
End Date:
28/02/2026
