Abstract
This project exploits SAMMY, a cutting-edge technology enabling the sequential isolation of chromatin fractions based on DNA accessibility, to explore bacterial dormancy in the major human pathogen Helicobacter pylori (HP). Dormant coccoid forms of HP contribute to persistence, antibiotic treatment failure, and constitute a major threat to public health worldwide. SAMMY will be applied for the first time to a bacterial system to generate an OMICS-level map of nucleoid conformations under various growth and stress conditions. We will combine whole transcriptome analysis and investigate the role of crucial transcription factors of HP in chromatin organization and coccoid formation. This innovative approach will uncover key structural and regulatory features of the HP nucleoid, providing new insights into dormancy mechanisms and persistence, and paving the way for novel antimicrobial strategies.
Project details
Unibo Team Leader: Davide Roncarati
Unibo involved Department/s:
Dipartimento di Farmacia e Biotecnologie
Coordinator:
CNR - Consiglio Nazionale delle Ricerche(Italy)
Total Unibo Contribution: Euro (EUR) 92.179,00
Project Duration in months: 24
Start Date:
28/09/2023
End Date:
28/02/2026
