90171 - Biology and Genetics Lab-based Course (B)

Academic Year 2018/2019

  • Teaching Mode: Traditional lectures
  • Campus: Bologna
  • Corso: Single cycle degree programme (LMCU) in Medicine and Surgery (cod. 8415)

Learning outcomes

At the end of the course, the student has experienced the main methods of cell biology and general and molecular genetics applied to biomedical sciences. In particular, it knows the fields of application and the limits of the main techniques used in cell biology, with particular attention to cell cultures, biotechnologies and the use of transgenic animals. He has learned to recognize the elements of a gene in a real DNA sequence, is able to consult the major online molecular genetic banks; he has performed a simple DNA extraction, and has discussed the rational basis of the method; he has designed an experiment of polymerase chain reaction (PCR) to amplify DNA. He has learned to reconstruct the path from experimental data to building current biomedical knowledge to evaluate the methodological aspects of knowledge by taking a critical approach to the study.

Course contents

Gene study and database.

DNA extraction and electrophoresis.

Gene cloning: applications and technologies.

Zebrafish in functional genomics.

RNA interference: theory and applications.

The research in medicine: review of some papers.

Readings/Bibliography

Some articles and reviews from Medical Journals chosen by the teachers.

Teaching methods

Activities in lab. Virtual experiences, videos, teamwork and discussion of some topics.


Assessment methods

The principal topics developed during the practical activities could be subject of the oral review.

Teaching tools

Reviews, videos, software applications, digital platforms and teacher's original schemes and notes.


Links to further information

http://apollo11.isto.unibo.it/

Office hours

See the website of Lorenza Vitale