B9149 - TECNICHE DI ISTOPATOLOGIA (CE)

Learning outcomes

Upon completion of the course, students will have acquired knowledge of how to prepare and process histological and cytological specimens for optical microscopy. They will also gain an understanding of the principles of biopsy specimen preservation, including physical and chemical fixation, as well as the principles of optical microscopy and technologies and instrumentation in pathological anatomy. They will learn how to prepare histological specimens for examination, including embedding them in paraffin and resin, sectioning them, and performing basic staining. They will also learn about the methods used for anatomical and pathological diagnosis, including histochemistry, enzymohistochemistry, immunohistochemistry, immunofluorescence, and in situ staining. Finally, they will learn about the technical activities related to the dissection room.

Course contents

- Preservation of biopsy specimens: fixation using physical and chemical methods

- Principles of optical microscopy

- Technologies and instrumentation in pathological anatomy

- Preparation of histological specimens: embedding in paraffin wax, sectioning and basic staining for observation under a light microscope

- Methods for anatomical-pathological diagnosis: histochemistry, enzymohistochemistry, immunohistochemistry, immunofluorescence and in situ staining

Readings/Bibliography

Slides and notes taken during the lessons are essential for exam preparation.

Teaching methods

Lectures with the support of explanatory slides.

Assessment methods

Theoretical knowledge will be verified by an oral test. The evaluation will be expressed as a score out of 30 and will take into account the clarity and coherence of the presentation of the topic, the accuracy of the content and terminology used, and the ability to think critically. The grade obtained in this course will be combined with those obtained in the other two C.I. courses to determine the final grade.

1. The exam will be administered in person, except in cases of local health or flooding conditions, in risk areas as per the new regulations, or if the student is vulnerable (an assessment will be carried out by the SLD service). Each case will be assessed individually and, if the requirements are met, the exam may be taken via the TEAMS platform.

2. Students with SLD or temporary or permanent disabilities are recommended to contact the relevant university office in good time (https://site.unibo.it/studenti-con-disabilita-e-dsa/en). The Office will be responsible for proposing any adaptations to the students concerned, but these must be submitted for the teacher's approval at least 15 days in advance. The teacher will then evaluate them in relation to the course's educational objectives.

3. The use of AI is prohibited. Any use constitutes a violation of academic integrity.

Teaching tools

PC, projector

Office hours

See the website of Silvia Turroni