C8713 - ADVANCED TOPICS IN EUKARYOTIC GENE EXPRESSION

Learning outcomes

At the end of the course, the student: 1) can analyze, interpret, and make conclusions from research article data and use this knowledge to deepen his/her understanding of eukaryotic gene expression; 2) can explain many of the molecular mechanisms by which eukaryotic gene expression can be controlled; 3) knows how to design experimental strategies to address questions related to gene expression molecular mechanisms.

Course contents

Eukaryotes use multiple interconnected regulatory mechanisms to control gene expression. The module will provide a current understanding of some of these mechanisms through lectures supported by analysis and discussion of research articles and recent reviews. Topics that are likely to be addressed include: How nuclear architecture influences gene expression; how transcription is regulate by DNA elements; mechanisms of regulation by non-coding RNAs; the consequences when gene expression control mechanisms are defective. The mechanisms will be considered at the molecular level in a range of eukaryotic model organisms.

Antisense RNA, Genetic RNA interference (RNAi) by dsRNA in Caenorhabditis elegans, short interfering RNA (siRNA), Dicer, RNAi and small temporal RNA (stRNA), siRNA and miRNA functional asymmetry, Recognition of primary microRNAs (pri-miRNAs) by the Drosha-DGCR8 complex, Argonaute Proteins and RISC assembly, Piwi-interacting RNA (piRNA). Transcription and RNAi in the formation of heterochromatin, Gene regulation by long non-coding RNAs (lncRNAs) and their biological functions. Chromatin accessibility, Core Promoters, Enhancer Characterization, Chromosome Conformation Capture Technologies, Topologically Associating Domain (TAD), DNA Insulators, Transcriptional Silencers, Transcription Factors, Genome-Wide Association Study (GWAS)

Readings/Bibliography

Selected scientific articles and recent reviews chosen mong the most significant publications in the field of molecular biology.

Teaching methods

The two main teaching methods are classroom-based lectures and laboratory-based experimental activities; both are conducted in person. “Given the nature of the activities and teaching methods employed, participation in this course requires all students to complete Modules 1 and 2 via e-learning and to attend Module 3, which provides specific training on health and safety in study environments. Information regarding the dates and attendance requirements for Module 3 can be found in the dedicated section of the degree program’s website.”

Assessment methods

The exam consists of a written test with open-ended questions. Students have 120 minutes for the written test. The use of supporting materials such as textbooks, notes, or any type of computer media is prohibited during the test. The use of AI is prohibited for assessments. Any use constitutes a violation of academic integrity.

The maximum score achievable for providing all correct and complete answers is 30 cum laude. The test is passed with a minimum score of 18/30.

Students with learning disabilities or temporary or permanent disabilities, please contact the relevant University Office (https://site.unibo.it/studenti-con-disabilita-e-dsa/it ).

Students recognized as “working students”, please consult the dedicated website (https://www.unibo.it/en/study/guide-to-choosing-your-programme/balancing-study-and-work) to apply for this status and to learn about the available measures.

Teaching tools

Whiteboard; Slide and video projection

Collaborative group learning;

Experimental biomolecular laboratory activities.

Office hours

See the website of Davide Carlo Ambrosetti