84278 - Cellular Biology and Genetics Laboratory (G2)

Learning outcomes

Integrated Course: The Integrated Course in CELLULAR MOLECULAR BIOLOGY AND GENETICS aims to provide students with a comprehensive understanding of the structural and functional organization of eukaryotic cells, the principles of genetic inheritance, and the molecular mechanisms governing the regulation, analysis, and modulation of both stem and differentiated cell behaviors. Through a combination of theoretical lessons, critical evaluation of scientific literature, and hands-on laboratory training, the course offers a coherent framework linking fundamental concepts to experimental approaches relevant to biomedical research and clinical applications. Learning Activity: By the end of the CELLULAR BIOLOGY AND GENETICS course the student will be able to:- Define the structural components of eukaryotic cells including the membrane, organelles and cytoskeleton. Describe fundamental cellular processes, including cell movement, targeting and trafficking, cell-cell communication, cell cycle, cell division, and apoptosis. Describe the organization of the human genome and stucture and function of human chromosomes. Recognize the pattern of transmission of hereditary characters and exceptions to mendelian inheritance. Identify the types, causes and biological consequences of genetic variation, with due attention to individual, population and sex differences. Discuss the impact of evolution on human genetic traits and disease risk.

Course contents

Students have the chance to experience research first hand alongside experts in their fields. They utilize the main tools and techniques in molecular and cell biology including DNA extraction, cloning and sequencing. They can access the main biological databases for human research, design an experiment and discuss key steps, benefits and pitfalls in order to improve critical thinking skills.

Lab activities will consist in:

- learning the use of the main query commands of the online database Pubmed

- gene structure analysis (using the main biological databases)

- DNA extraction

- gene expression studies

- amplifying DNA: polymerase chain reaction (PCR)

-DNA sequence analysis

- journal club

Readings/Bibliography

Schemes and articles will be provided during the course.

Teaching methods

- Lab activities

- Virtual experiences, video, teamwork

- Discussion of some topics.

 

In consideration of the types of activities and teaching methods adopted, the frequency of this training activity requires all students to carry out modules 1 and 2 in e-learning mode and to participate in a third specific training module on safety and health in the places of study / internship. Indications on the dates and methods of attendance of the third module will be communicated to the students by the training centers.

Assessment methods

Discussion at the end of each activity. Lab activities can be used by teachers to enhance intended learning outcomes of the integrated course.

Cellular Molecular Biology and Genetics (I.C.)

Block 1_Cellular Biology and Genetics + Lab: the final examination consists of an oral dissertation (two questions randomly selected) to assess achievement of the skills detailed in the learning outcomes. The student is asked to demonstrate skills in terms of synthesis, analysis and critical appraisal of the topics covered in the course. The maximum score obtainable is equal to 30 cum laude. The exam is considered passed with a minimum score of 18/30.

Block 2_Molecular Biology: the students will be evaluated by written exam. The written test consists of 31 multiple-choice questions, with only one correct answer per question. Students will receive 1 point for each correct answer, and 0 points for incorrect or missing answers. The time available to students for the written test is 30 minutes. During the test, the use of support materials, such as textbooks, notes, computer supports, is not allowed. The maximum score obtainable by providing all correct answers is equal to 30 cum laude. The test is considered passed with a minimum score of 18/30.

Each block of the Integrated Course (I.C.) in Cellular and Molecular Cell Biology and Genetics will receive an evaluation, and the final decision on the grade will be taken collegially by the teaching staff, taking into account the weighted average.

Students with learning disorders and\or temporary or permanent disabilities: please, contact the office responsible (https://site. unibo.it/studenti-con- disabilita-e-dsa/en/for- students [https://site.unibo.it/studenti-con-disabilita-e-dsa/en/for-students] ) as soon as possible so that they can propose acceptable adjustments. The request for adaptation must be submitted in advance (15 days before the exam date) to the lecturer, who will assess the appropriateness of the adjustments, taking into account the teaching objectives.

Teaching tools

- Slides with images and schemes.

- Databases and tutorials available online and analysis softwares.

- Selection of scientific articles.

Office hours

See the website of Maria Chiara Pelleri