- Docente: Stefania Rapino
- Credits: 3
- SSD: CHIM/02
- Language: English
- Teaching Mode: Traditional lectures
- Campus: Bologna
- Corso: Second cycle degree programme (LM) in Molecular and Cell Biology (cod. 5825)
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from Nov 05, 2025 to Dec 19, 2025
Learning outcomes
At the end of the module the student is familiar with modern chemical and physical technologies employed for the study and diagnosis of cancer and their applications to cancer biology; She/He gains knowledge about the techniques which are well assessed in the field as well as about the most innovative ones as emerging from the recent scientific literature. The student is able to critically evaluate and plan the employment of these methodologies in cancer biology research.
Course contents
In order to achieve the learning outcomes of the course the program of the teaching unit will focus on the following topics:
i) 3D cell cultures in cancer research: organoids, organ on chips and microfluidics, 3D bioprinting;
ii) Biosensors and other approaches for the study of cell metabolism (cell populations and at a single cell level);
iii) Real-Time cell metabolic analysis;
iv) in situ spectroscopic techniques;
v) Methods and procedures for proteomic analyses;
vi) State-of-the-art methods and techniques for sequencing and single cell sequencing; In the past twenty years the study and diagnosis of cancer has drastically changed, moving from a morphology-based analysis to a molecular one, starting from the use of conventional sequencing technologies till the introduction of next generation sequencing in the routinary practice. We will here focus on: basic sequencing chemistry from Sanger to NGS, advantages and disadvantages of newer technologies, data analysis, interpretation, validation and reporting, applications of DNA- and RNA-based technologies.
vii) Atomic force microscopies and other scanning probe microscopies in cancer research;
viii) Techniques for the high-sensitivity detection of tumor biomarkers;
ix) Positron Emission Tomography (PET) and Functional Magnetic Resonance Imaging (MRI), isotopic labeling and integrated approaches, e.g. radiomics.
x) Artificial intelligence in cancer research and diagnosis.
Readings/Bibliography
Lecture notes and teacher sllides
Papers from scientific literatureTeaching methods
Frontal Lectures on the program topics; Seminars on specific and innovative arguments.
Discussions and brain storming involving the whole class will be a fundamental teaching approach.
Assessment methods
The Cancer Biology exam will consist of a single written test covering all three modules.
The test will include 30 focused multiple-choice questions (10 per module) and 3 open-ended questions (one per module).
The final grade will be calculated as follows:
- 1/4 of the total grade will be based on the multiple-choice questions
- 1/4 of the total grade will be assigned to each open-ended question
The final grade, expressed out of 30, will be a single overall score for all modules.Students will have two and a half hours to complete the entire written test.
Students have the right to reject the proposed positive grade once (in accordance with the University Teaching Regulations, ART.16, paragraph 5).
Students with learning disorders and\or temporary or permanent disabilities: please, contact the office responsible (https://site.unibo.it/studenti-con-disabilita-e-dsa/en ) as soon as possible so that they can propose acceptable adjustments. The request for adaptation must be submitted in advance (15 days before the exam date) to the lecturer, who will assess the appropriateness of the adjustments, taking into account the teaching objectives.
Students recognized as “working students”: please consult the dedicated website (https://www.unibo.it/en/study/guide-to-choosing-your-programme/balancing-study-and-work ) to apply for this status and to learn about the available measures.
Teaching tools
Teaching is mainly carried employing PC, beamer projector, Power Point presentations. Discussion on papers from scientific literature.
Office hours
See the website of Stefania Rapino
SDGs



This teaching activity contributes to the achievement of the Sustainable Development Goals of the UN 2030 Agenda.