- Docente: Pierluigi Strippoli
- Credits: 5
- SSD: BIO/13
- Language: English
- Moduli: Pierluigi Strippoli (Modulo 1) Pierluigi Strippoli (Modulo 2)
- Teaching Mode: Traditional lectures (Modulo 1) Traditional lectures (Modulo 2)
- Campus: Bologna
- Corso: Second cycle degree programme (LM) in Medical Biotechnology (cod. 9081)
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from Oct 29, 2024 to Dec 18, 2024
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from Oct 30, 2024 to Nov 28, 2024
Learning outcomes
Discuss the structure, organization, variability, genetic information and gene families of the human genome. Critically appraise the rational basis of the main molecular and computational biology methods used for the study of genomes, including both classical and next-generation approaches. Consult and study bibliographic sources to self-update on innovative genomic topics and techniques. Use common software tools for genetics and genomics, and apply them to the biomedical field.
Course contents
1. Human Genomics and Post-Genomics
2. Bibliography and Genomics databases
3. Methods for studying nucleic acid
4. Nucleic Acids Hybridization (Southern Blot, Northern Blot, Microarray)
5. Polymerase Chain Reaction (Kinetics, Parameters, Design)
6. cDNA, EST and genomic Libraries
7. Nucleic Acids Sequencing and Sequence Analysis
8. Computational and System Biology - Trisomy 21
Laboratory (15 hours)
1. Advanced use of bibliography and genomics databanks
2. Gene structure - NCBI Gene database
3. PCR primer design
4. Sequence analysis
5. Documentary and discussion
Readings/Bibliography
Website: http://apollo11.isto.unibo.it/
Website: http://apollo11.isto.unibo.it/biotech/en/Biotech.htm
Teaching materials will be made available through both public websites and Student access to the "Virtuale" platform.
Teaching methods
In consideration of the type of activity and the teaching methods adopted, the attendance of this training activity REQUIRES the prior participation of all Students in the Training Modules 1 and 2 about safety in the study places, in e-learning mode.
Assessment methods
16 multiple choice questions on the treated topics, in person. Attendance to learning activities is mandatory; the minimum attendance requirement to be admitted to the final exam is 60% of lessons and laboratories.
Teaching tools
Availability of personal computers (iMac) in the "Auletta Multimediale".
Office hoursSee the website of Pierluigi Strippoli [https://www.unibo.it/sitoweb/pierluigi.strippoli/en]
Office hours
See the website of Pierluigi Strippoli