76166 - Molecular Biology

Learning outcomes

Define the mechanisms involved in the replication, repair, transcription and translation of information encoded in nucleic acids. Describe the molecular mechanisms that regulate gene expression, including epigenetics. Describe the molecular mechanisms of cell fate determination and differentiation.

Course contents

- Introduction to the field of Molecular Biology - DNA structure and function

Presentation of the course. The central Dogma of Molecular Biology.  Initial discoveries of the Molecular Biology field: DNA structure and function. Nucleotides, covalent and hydrogen bonds, DNA orientation. DNA conformations.

- Molecular and cellular mechanisms of cell renewal in organ development and homeostasis

Embryonic and adult stem cells, stem cell potency and differentiation. Adult stem cells in tissues with high cell renewal (i.e. bone marrow, intestine, skin...). Adult stem cells in tissues with low cell renewal (i.e skeletal muscle, brain). Cardiac stem and progenitor cells during embryonic development. Molecular mechanisms of stem cell regulation: extracellular matrix, soluble factors, communications between adjacent cells and mechanical stimuli. The role of transcription factors in cell differentiation.

- Molecular tools for studying cell renewal based on the analysis of DNA replication.

Nucleoside analogue incorporation assay for evaluation of cell proliferation. BrdU incorporation assay in vitro and in vivo for the evaluation of tissue renewal and regeneration. 14C incorporation for evaluation of DNA replication in tissues with very limited renewal, such as the heart and the brain.

- Site-specific recombinase technologies for gene knock-out/knock-in.

Recombinases. Structure and mechanism of action of Cre recombinase. Cre-Lox system for tissue-specific gene knock-out and knock-in. Inducible recombinases.

- Lineage tracing analysis to follow the fate of individual cells and their progeny.

Lineage tracing analysis: basic concepts and application examples for the evaluation of the contribution of cell populations during tissue regeneration.

- Advanced molecular tools for time-controlled gene expression.

Bacterial Tetracycline resistance mechanism: the "Tet Repressor" protein (TetR). Tet-OFF / Tet-ON system for inducible gene expression.

- Adult stem cell differentiation and mature cell dedifferentiation: molecular mechanisms and applications to regenerative medicine.

Adult stem cell-based therapies for regenerative medicine. The controversy of adult stem cell therapies for cardiac regeneration: the emerging consensus based on lineage tracing analyses. Cell dedifferentiation markers and features. Cell signalling pathways in cell dedifferentiation. Cell dedifferentiation in tissue regeneration.

- Cell reprogramming

 Cell reprogramming from somatic cells to induced pluripotent stem cells (IPS). Molecular factors for in vitro cell differentiation of induced pluripotent stem cells (IPS) and embryonic stem cells (ES). Applicative aspects of induced pluripotent stem cells (IPS) and embryonic stem cells (ES) in regenerative medicine. Direct reprogramming of somatic cell to specific cell types (transdifferentiation).

 

Readings/Bibliography

The course is based on the presentation of the material that will be supplied to the students via Virtual Learning Environment portal.

Teaching methods

• Frontal lessons through computer-assisted presentations
• Critical discussion of scientific articles.
• Tests at the end of the lessons will be used to support learning.
  

Attendance to this learning activity is mandatory; the minimum attendance requirement to be admitted to the final exam is 60% of lessons. For Integrated Courses (IC), the 60% attendance requirement refers to the total amount of I.C. lessons. Students who fail to meet the minimum attendance requirement will not be admitted to the final exam of the course and will have to attend relevant classes again during the next academic year. Professors may authorize excused absences upon receipt of proper justifying documentation, in case of illness or serious reasons. Excused absences do not count against a student’s attendance record to determine their minimum attendance requirement.

Assessment methods

The students will be evaluated by oral exams on the basis of two or more questions on the topics described during the course. The first question/topic will be chosen by the student, whereas the other questions/topics will be chosen by the Professor. At the end of the examination, the Professor will score the performance of the student using a number ranging from 18 to 30, according to the following criteria:

18 - 19: Preparation on a very limited number of topics covered in the course and analytical skills that emerge only with the help of the teacher, expressed in an overall correct language;

20 - 24: Preparation on a limited number of topics covered in the course and ability to autonomous analysis only on purely executive matters, expression in correct language;

25 - 29: Preparation on a large number of topics covered in the course, ability to make independent choices of critical analysis, mastery of specific terminology;

30 - 30 cum laude: Substantially exhaustive preparation on the topics addressed in the course, ability to make independent choices of critical analysis and connection, full mastery of specific terminology and ability to argue and self-reflection.

The final mark of the Integrated Course (I.C.) will be determined as weighted average mark (weights are the relative course credits).

Below the applied formula:

Final score I.C. = [(Cellular Biology and Genetics * 7) + (Cellular Biology and Genetics Laboratory * 1) + (Molecular Biology * 2)] / 10

For example:

Cellular Biology and Genetics (7 credits): 28/30

Cellular Biology and Genetics Laboratory (1 credits): 29/30

Molecular Biology (2 credits): 30/30

Weighted average mark: [(28*7)+(29*1)+(30*2)] / 10 = 28.85

Final mark: 29/30

For more info: https://www.unibo.it/en/teaching/course-timetable-and-exams/about-exams

Teaching tools

• Powerpoint presentations
• Scientific videos
• Scientific articles and reviews

All the material will be available to the Students via Virtual Learning Environment portal 

Office hours

See the website of Gabriele Matteo D'Uva