- Docente: Giulia Andreani
- Credits: 2
- SSD: BIO/12
- Language: Italian
- Teaching Mode: Traditional lectures
- Campus: Bologna
- Corso: Single cycle degree programme (LMCU) in Veterinary Medicine (cod. 8617)
Learning outcomes
At the end of the course the student knows the most important laboratory tests and biochemical techniques applied in the veterinary clinical fields.
Course contents
General laboratory concepts: descriptive statistics, reference values, quality control.
Sampling techniques: anticoagulants, preanalytic errors, centrifugation.
Artifacts in biochemical determinations: hemolysis, causes of color interference, lipemia.
Urinalysis: color and turbidity, chimical analysis, measurement of specific gravity, microscopic analysis of the sediment. Proteinuria: urine-creatinine ratio, urine electrophoresis. Urinary disorders.
Complete blood count: hemocitometer, automated hematology cell counters, hemoglobin concentration, erythrocyte indices. Blood smear analysis: leukocyte estimation, platelet estimation.
Autoanalyzer in clinical biochemistry: calibration, sensibility and sensitivity of a method;Ultraviolet-Visible and Visible Spectrophotometry.
Enzymes in veterinary medicine: enzymatic biomarkers in hepatic disorders, muscolar enzymatic biomarkers.
Non enzymatic assays for monitoring hepatic and renal function.
Glucidic metabolism: esokinse assay for glicaemia determination; follow up with laboratory test of the diabetic patient
Serum total protein analysis: protein electrophoresis, causes of altered electrophoretic patterns.
Readings/Bibliography
B.F.Feldman, J.G. Zinkl , N.C. Jain: Schalm's Veterinary Hematology. Fifth Edition. Edit by Lippincott Williams & Wilkins; A. Gaw et al., Biochimica clinica, Elsevier Masson, Milano, 2007.
Meyer D. J. , Harvey J. W. - Medicina di laboratorio veterinaria: interpretazione e diagnosi- Delfino Editore, 2007, 3° edizione.
E. Villiers, L. Blackwood – Gli esami di laboratorio. Edizione italiana a cura di Saverio Paltrinieri. UTET editore 2006.
J.J. Kaneko, J.W. Harvey, M.L. Bruss – Clinical biochemistry of domestic animals. AP Press, sixth edition, 2008.
Teaching methods
Ex cathedra and practical work. Practical work is be considered very important. Problem solving.
Assessment methods
Written examination
Teaching tools
Powerpoint presentations will illustrate the course contents. The practical work in laboratory is considered very important. Problem solving through evidence-based approach
Office hours
See the website of Giulia Andreani