- Docente: Gianandrea Pasquinelli
- Credits: 2
- SSD: MED/05
- Language: Italian
- Teaching Mode: Traditional lectures
- Campus: Bologna
- Corso: First cycle degree programme (L) in Techniques in the Biomedical Laboratory (for certified professional technicians in the biomedical laboratory) (cod. 0170)
Learning outcomes
The Course is aimed to illustrate: i) instruments and techniques applied to the field of microscopic research, ii) theoretical considerations on the different classes of microscopes (optical, electron, analytical and probe-based), iii) general and specific techniques applied to Cytopathology and Ultrastructural Pathology.
Course contents
From Malpighi observations to the world of nanomorphology. 1. Introduction 2. Light microscopy and the Abbe's diffraction law 3. History of electron microscopes (TEM) 4. TEM - Principles of operation and Examples of biological applications 5. The Scanning Electron Microscope (SEM) - history. The principle of scanning 6. Operating modalities 7. Preparation techniques for SEM I; Examples of biological applications 8. Preparation techniques for SEM II; Examples of biological applications 9. Analytical microscopy. 10 X ray microanalysis Examples of biological applications 11. EELS/EFTEM - Examples of biological applications 12. Immunogold labeling. 13. Cryoelectron microscopy. 14. New microscopies. Tomography. Enviromental SEM and Scanning Probe Microscopes (AFM/STM/SNOM)
Readings/Bibliography
Microscopie in Biologia e Medicina. C Scala, G Pasquinelli, G Cenacchi. CLUEB Bologna
Teaching methods
Strategies to solve specific issues will be discussed.
Assessment methods
Oral assessment.
Teaching tools
Videoprojection, PC, PPT slides
Office hours
See the website of Gianandrea Pasquinelli