- Docente: Maria Laura Bacci
- Credits: 3
- SSD: VET/02
- Language: Italian
- Teaching Mode: Traditional lectures
- Campus: Bologna
- Corso: First cycle degree programme (L) in Biotechnology (cod. 8005)
Learning outcomes
The student must have knowledge of male and female gametogenesis in order to know and understand principal biotechnologies, comprising transgenesis, applied to male and female gametes and embryos of domestic, terrestrial and aquatic, species.
Course contents
Outline of reproductive function regulation. Reproductive hormones (hypothalamus-hypophysis-gonad axis) and endocrinological function of gonads
Female. Ovarian function: oogenesis, folliculogenesis, ovulation and corpus luteum.
Male. Testicle function: spermatogenesis. Male gametes: characteristics and function.
Embryos: Fertilization. First phases of embryo development till blastocyst stage.
Reproductive biotechnologies applied to domestic, terrestrial and aquatic, species.
Female gamete: in vivo and ex vivo oocyte collection; oocyte qualitative evaluation; techniques for in vitro growing oocyte; oocyte in vitro maturation, micromanipulation and cryoconservation.
Male gamete: in vivo and ex vixo (epididimal) spermatozoa collection; quali-quantitative semen analysis, dilution and cryoconservation of spermatozoa by refrigeration/freezing/vitrification. Functional characteristic evaluation of spermatozoa. Fertilization techniques with frozen or fresh semen, artificial insemination, laparotomic/laparoscopic intrauterine insemination. Spermatozoa sexing.
Embryos: surgical and non surgical in vivo collection, in vitro production by in vitro fertilization; in vitro colture; embryotransfer; micromanipulation: splitting, nuclear transfer; cloning; stem cell; sexing. Transgnenesis.
Readings/Bibliography
Lesson notices; Reproduction in Farm Animals 7th edition, E. S. E. Hafez, B. Hafez (Editors) 2000 Lippincott Wlliams and Wilkins Dukes, Fisiologia degli animali domestici, IDELSON-GNOCCHI, Napoli (2002).
Teaching methods
Lectures
Assessment methods
The assessment method will be an oral discussion on lectures.
Teaching tools
Videoprojector, overhead projector, PC
Office hours
See the website of Maria Laura Bacci