- Docente: Alessandra Tolomelli
- Credits: 3
- SSD: CHIM/06
- Language: Italian
- Teaching Mode: Traditional lectures
- Campus: Bologna
- Corso: Second cycle degree programme (LM) in Molecular and industrial biotechnology (cod. 8022)
Learning outcomes
At the end of the course, the student will know enzymatic mechanisms and will be able to apply them to the solution of biotechnological problems and to the design of the asymmetric synthesis of novel organic compounds. The students will know - classification of enzymes and mechanism of the six principal classes and of the main sub-classes; - the stereochemical aspects of biocatalysis; - the use of biocatalysts in organic synthesis. In particular, the student will be able to plan different aternative synthetic strategies using enzymes as biocatalysts.
Course contents
Hydrolysis and formation of phosphate esters. Kinases
Aldolases (class I and classII), FDP aldolases
Isomerase (EC5). Mechanism and industrial application.
Biological oxidations (EC1)
NAD and FAD
Stereospecific reactions with NAD
Lattate dehydrogenase, alcohol dehydrogenase, aldehyde dehydrogenase.
Readings/Bibliography
K. Faber, Biotransformations in Organic Chemistry, Springer editor.
W. Gerhartz, Enzymes in Industry, VCH editor
Abeles, R. H.; Frey, P. A.; Jencks, W. P. Biochimica, PIccin editore.
Biocatalysis - Fundamentals and applications, Wiley VCH editor.
Assessment methods
Oral discussion
Teaching tools
Lessons
Office hours
See the website of Alessandra Tolomelli