84282 - Histology Laboratory

Course Unit Page

  • Teacher Mattia Lauriola

  • Credits 1

  • SSD BIO/17

  • Teaching Mode Traditional lectures

  • Language English

Academic Year 2017/2018

Learning outcomes

Identify distinct cytotypes and recognize normal tissue morphology by means of optical microscopy.

Course contents

Students will apply histotechnology theory to laboratory skills

Introduction on the histotechnology by detailing tissue preparation procedures, techniques for essential histological identifications by light microscope, commons staining and relevant protocols for cell schape and size identification.

Epithelial tissue

Distinguish between, simple, stratified, and pseudostratified epithelia

Cell shape characteristic of squamous, cuboidal, columnar, and transitional epithelia

Identification of surface specializations

Organization and function of endocrine and esocrine tissues.

Connective Tissue

Identification of the structural organization both of fibers in the extracellular matrix (ECM) and the cells residing within connective tissue

Loose and dense connective tissue using the light microscope

White and brown adipose tissue

Cartilage classification (hyaline, fibrous and elastic)

Bone tissue, Intramembranous and Endochondral ossification

Observe and describe the processes of bone remodeling and bone growth.

Describe the calcification processes of cartilage and bone.

Blood

Identify the component cells of a blood smear

Identify the hematopoietic precursor cells of a typical bone marrow smear

Distinguish the different classes of white blood cells

Muscle Tissue

Identify the histological landmarks of skeletal muscle

Contrast the structure and function of skeletal, smooth, and cardiac muscle tissue.

Identify morphological differences in smooth muscle across tissues.

Explain the structure and function of the intercalated disc

Nervous System

Histological and structural features of neurons

Microscopic structure of a nerve fiber, including the structure of the myelin sheath and connective tissue layers.

Identify the four types of glial cells, their structures, and their functions.

Readings/Bibliography

to be defined with the students

Teaching methods

Projection of several prototype of histological preparations followed by student driven autonomous recognition and discussion. No more then 25 students for class are allowed.

Assessment methods

The final test consists of identification by light microscope of randomly selected histological preparations

Teaching tools

Histological preparations on the slide.

Powerpoint presentations

Mono and binocular optical microscopes for autonomous evaluation of tissues

5-head optical microscope for histologic tutoring of small groups of students

optical microscope connected to a computer projector for class discussion of tissue’s features.

Virtual histological preparations are available to be displayed with computer tools and microscope simulation programs.

Office hours

See the website of Mattia Lauriola