Assessment of shelf-life of rabbit meat for fattening fed
with feed enriched with antioxidants. Determination of mycotoxins
potentially present in the matrices investigated. Setting up
of triangular test for evaluating the sensory discriminative
capacity of "enriched" meat.
Bacterial canker disease of actinidia caused by Pseudomonas
syringae pv. actinidiae: development of defence strategies. A)
Endophytic colonization of Psa in actinidia adult plants; B) S
urvival of Psa in micropropagated shoots of Actinidia
deliciosa cv. Hayward; C) R ing test analysis to set up an
harmonized and validated protocol for the diagnosis of Psa; D) D
evelopment of a method to evaluate the stomata dynamic involved in
the initial immediate immune response of actinidia plants
against Psa.
Antibacterial activity of essential natural oils from different Monarda medicinal
plants
Ochratoxin production in strains of Aspergillus
ochraceus, agent of food spoilage, and assessment of
antimicrobial effect of essential oils and organic acids in
vitro and in vivo.
Molecular bases of the fire blight resistance in pear plant.
Study of the epiphytic and endophytic E. amylovora
populations and of the expression of virulence genes in symptomatic
and asymptomatic pear plants.
Molecular epidemiology of the Erwinia amylovora
populations in the Po Valley.
Quorum quenching of phytopathogenic bacteria and induced
resistance in host plants by synthetic furanone treatments.
Use of electronic nose for the diagnosis of the bacterial plant
diseases. The use of the electronic nose (e-nose) for the diagnosis
of bacterial diseases has recently been developed in UK for the
diagnosis of ring rot, caused by Clavibacter michiganense
subsp. sepedonicus and brown rot, caused by Ralstonia
solanacearum. The use of the e-nose could also be proposed for
other pathosystems. For instance it could be applied to the seeds
during the hydration stage where the plant and the associated
microorganisms released various substances, some volatile.
Endophytes of grapevine as possible agents of biocontrol and
biofertilizer.
Assessment of shelf-life of rabbit meat for fattening fed
with feed enriched with antioxidants. Determination of mycotoxins
potentially present in the matrices investigated. Setting up
of triangular test for evaluating the sensory discriminative
capacity of "enriched" meat.
Advancing knowledge on the interaction between P.
syringae pv. actinidiae and actinidia plant at
biomolecular and epidemiological level. The study of the pathogen
and its close interaction with the host, will provide the basic
knowledge to set up and test biocontrol methods necessary to limit
the spread of the disease. The development of appropriate
diagnostic methods will allow the determination of the health
status of planting material in order to avoid further dissemination
of the pathogen.
Ochratoxin production in strains of Aspergillus
ochraceus, agent of food spoilage, and assessment of
antimicrobial effect of essential oils and organic acids in
vitro and in vivo.
Antibacterial activity of essential natural oils from different Monarda medicinal
plants
Study of the incidence and severity of the disease caused by a
selected E. amylovora strains by phytopathometric analysis.
The symptomatic and asymptomatic pear plants previously inoculated
will be kept to study the endophytic population level of the
pathogen re-isolating it on selective-medium and using the Real
Time PCR (SYBR Green). Besides, to confirm the results of the Real
Time PCR and to evaluate the endophytic distribution of the
microorganism along the xylem vessels an E. amylovora strain
will be marked with the gfp reporter gene associated to a
virulence gene of the pathogen (dspE, that is needed to the
synthesis of an important factor which interferes with the
host-plant immune system). On the same plants, the epiphytic level
of E. amylovora population directly on leaves will be
evaluated using the leaf imprint method that needs the use of a
rifampicin resistant strain; moreover, using the gfp
reporter gene it will be possible to verify the pathogen capacity
in surviving on the leaves of the host-resistant plants.
Molecular epidemiology of the Erwinia amylovora
populations in the Po Valley.
Quorum quenching of phytopathogenic bacteria and induced
resistance in host plants by synthetic furanone treatments.
Use of electronic nose for the diagnosis of the bacterial plant
diseases. The use of the electronic nose (e-nose) for the diagnosis
of bacterial diseases has recently been developed in UK for the
diagnosis of ring rot, caused by Clavibacter michiganense
subsp. sepedonicus and brown rot, caused by Ralstonia
solanacearum. The use of the e-nose could also be proposed for
other pathosystems. For instance it could be applied to the seeds
during the hydration stage where the plant and the associated
microorganisms released various substances, some volatile.
Isolation of a large number of bacterial endophytes from
grapevines grown in Italy. Preliminary classification of all
isolated bacterial endophytes to be screened for their in
vitro anti-fungal activity. All the endophytes that display
significant antifungal activity in vitro will be further
classified and tested for colonization of grapevine plantlets and
systemic movement within the grapevine. The few endophytes which
display significant anti-fungal activity and which efficiently
colonize grapevine plantlets, will be assessed for their biocontrol
and biofertilizer abilities.