Foto del docente

Paola Minardi

Assistant professor

Department of Agricultural and Food Sciences

Academic discipline: AGR/12 Plant Pathology

Research

Keywords: avirulence genes mycotoxins determination of mycotoxins by liquid chromatography and by mass spectrometry food and feed safety environmentally-safe strategies to bacterial disease control induced resistance agent of biocontrol organic acid and essential oils furanones quarantine phytopathogenic bacteria plant-bacteria interactions antioxidants

Assessment of shelf-life of rabbit meat for fattening fed with feed enriched with antioxidants. Determination of mycotoxins potentially present in the matrices investigated. Setting up of triangular test for evaluating the sensory discriminative capacity of "enriched" meat.

Bacterial canker disease of actinidia caused by Pseudomonas syringae pv. actinidiae: development of defence strategies. A) Endophytic colonization of Psa in actinidia adult plants; B) S urvival of Psa in micropropagated shoots of Actinidia deliciosa cv. Hayward; C) R ing test analysis to set up an harmonized and validated protocol for the diagnosis of Psa; D) D evelopment of a method to evaluate the stomata dynamic involved in the initial immediate immune response of  actinidia plants against Psa.

Antibacterial activity of essential natural oils from different Monarda medicinal plants

Ochratoxin production in strains of Aspergillus ochraceus, agent of food spoilage, and assessment of antimicrobial effect of essential oils and organic acids in vitro and in vivo.

Molecular bases of the fire blight resistance in pear plant. Study of the epiphytic and endophytic E. amylovora populations and of the expression of virulence genes in symptomatic and asymptomatic pear plants.  

Molecular epidemiology of the Erwinia amylovora populations in the Po Valley.

Quorum quenching of phytopathogenic bacteria and induced resistance in host plants by synthetic furanone treatments.

Use of electronic nose for the diagnosis of the bacterial plant diseases. The use of the electronic nose (e-nose) for the diagnosis of bacterial diseases has recently been developed in UK for the diagnosis of ring rot, caused by Clavibacter michiganense subsp. sepedonicus and brown rot, caused by Ralstonia solanacearum. The use of the e-nose could also be proposed for other pathosystems. For instance it could be applied to the seeds during the hydration stage where the plant and the associated microorganisms released various substances, some volatile.

Endophytes of grapevine as possible agents of biocontrol and biofertilizer.

 



Assessment of shelf-life of rabbit meat for fattening fed with feed enriched with antioxidants. Determination of mycotoxins potentially present in the matrices investigated. Setting up of triangular test for evaluating the sensory discriminative capacity of "enriched" meat.

Advancing knowledge on the interaction between P. syringae pv. actinidiae and actinidia plant at biomolecular and epidemiological level. The study of the pathogen and its close interaction with the host, will provide the basic knowledge to set up and test biocontrol methods necessary to limit the spread of the disease. The development of appropriate diagnostic methods will allow the determination of the health status of planting material in order to avoid further dissemination of the pathogen.

Ochratoxin production in strains of Aspergillus ochraceus, agent of food spoilage, and assessment of antimicrobial effect of essential oils and organic acids in vitro and in vivo.

Antibacterial activity of essential natural oils from different Monarda medicinal plants

Study of the incidence and severity of the disease caused by a selected E. amylovora strains by phytopathometric analysis. The symptomatic and asymptomatic pear plants previously inoculated will be kept to study the endophytic population level of the pathogen re-isolating it on selective-medium and using the Real Time PCR (SYBR Green). Besides, to confirm the results of the Real Time PCR and to evaluate the endophytic distribution of the microorganism along the xylem vessels an E. amylovora strain will be marked with the gfp reporter gene associated to a virulence gene of the pathogen (dspE, that is needed to the synthesis of an important factor which interferes with the host-plant immune system). On the same plants, the epiphytic level of E. amylovora population directly on leaves will be evaluated using the leaf imprint method that needs the use of a rifampicin resistant strain; moreover, using the gfp reporter gene it will be possible to verify the pathogen capacity in surviving on the leaves of the host-resistant plants.

Molecular epidemiology of the Erwinia amylovora populations in the Po Valley.

Quorum quenching of phytopathogenic bacteria and induced resistance in host plants by synthetic furanone treatments.

Use of electronic nose for the diagnosis of the bacterial plant diseases. The use of the electronic nose (e-nose) for the diagnosis of bacterial diseases has recently been developed in UK for the diagnosis of ring rot, caused by Clavibacter michiganense subsp. sepedonicus and brown rot, caused by Ralstonia solanacearum. The use of the e-nose could also be proposed for other pathosystems. For instance it could be applied to the seeds during the hydration stage where the plant and the associated microorganisms released various substances, some volatile.

Isolation of a large number of bacterial endophytes from grapevines grown in Italy. Preliminary classification of all isolated bacterial endophytes to be screened for their in vitro anti-fungal activity. All the endophytes that display significant antifungal activity in vitro will be further classified and tested for colonization of grapevine plantlets and systemic movement within the grapevine. The few endophytes which display significant anti-fungal activity and which efficiently colonize grapevine plantlets, will be assessed for their biocontrol and biofertilizer abilities.