24024 - Clinical Pathology Techniques

Academic Year 2012/2013

  • Docente: Davide Trerè
  • Credits: 1
  • SSD: MED/05
  • Language: Italian
  • Teaching Mode: Traditional lectures
  • Campus: Bologna
  • Corso: First cycle degree programme (L) in Techniques in the Biomedical Laboratory (for certified professional technicians in the biomedical laboratory) (cod. 0170)

Learning outcomes

To allow the students to acquire the fundamental notions for a critical evaluation and interpretation of the results of the main techniques employed in clinical pathology laboratory.

Course contents

- Techniques in laboratory medicine. Monoclonal and polyclonal antibodies; precipitation reactions (double immunodiffusion, radial immunodiffusion, immunoelectrophoresis, nephelometry); agglutination reactions (Coombs' direct and indirect tests, Waaler Rose test); complement fixation test; radioisotopic and non-radioisotopic immunometric methods; immunofluorescence; flow cytometry.

- Blood gas analysis for the diagnosis of acid-base disorders. Respiratory and metabolic acidosis and alkalosis; blood gas analysis in the simple and complex disruptions of acid-base balance.

- Tumor markers. Definition and classification; diagnostic specificity and sensibility; the tumor markers in the clinical practice; intermediate filaments as markers of differentiation; leukocytic antigens and the CD system.

Readings/Bibliography

- Widmann: Interpretazione clinica degli esami di laboratorio. Ronald A. Sacher e Richard A. McPherson. 2001 Undicesima edizione. Mc Graw Hill libri Italia (Milano)

- Joel D. Hubbard: A concise review of Clinical laboratory science. Williams & Wilkin

- Giorgio Federici: Medicina di Laboratorio. 2008 Terza edizione. Mc Graw Hill libri Italia (Milano)

Teaching methods

Frontal lessons

Assessment methods

Oral or quiz examination

Teaching tools

Office hours

See the website of Davide Trerè