- Docente: Gian Paolo Bagnara
- Credits: 3
- SSD: BIO/17
- Language: Italian
- Teaching Mode: Traditional lectures
- Campus: Bologna
- Corso: Second cycle degree programme (LS) in Biotechnology in Medicine (cod. 0239)
Learning outcomes
This teaching will provide: the general knowledge on human
embryology, progress report about embryonic stem cells and human
pluripotent mesenchymal stem cells, in particular the molecular
mechanisms on in vitro differentiation towards osteogenic,
chondrogenic steatogenic, hemo angiogenic, myogenic, myocardiogenic
and neurogenic lineages.
Lab practical (cell cultures and molecular biology) will complete
this training
Course contents
• Preliminare lesson: extraembryonic tissues for isolatine
pluripotent human mesenchymal stem cells.
• First and second weeks of the human prenatal development:
proliferative stage, bilaminar disc, primitive placenta
circulation.
• Third and fourth weeks of the human prenatal development:
gastrulation, trilaminar embryo, somite and neural tube
development, embryo folding.
• Fetal membranes: placenta, amnion, corion; in vitro cell
culture.
• Embryonic stem cells and adult stem cells
• Fetal hematopoiesis and bone marrow congenital dysplasias
• Osteogenesis and chondrogenesis in vitro
• Muscle development and myogenesis in vitro
Lab program
• Isolation of mesenchymal stem cells in vitro
from human placenta, amnion and corion: observation of placenta
structure, membrane separation, tissue digestion, plating of cell
suspension and biopsy
• Cytofluorimetric characterization by means
monoclonal antibodies
• Induction to different lineages
Readings/Bibliography
Barbieri, Carinci "EMBRIOLOGIA" casa editrice Ambrosiana
Moore, Persaud "LO SVILUPPO PRENATALE DELL'UOMO" casa editrice
EdiSES
Teaching methods
The course has two parts, 16 hours on theory and 15 hours practical
Assessment methods
this teaching is one part of the course on human reproduction and
genetic bases of hereditary diseases. the oral exam is
inseparabile from the other parts.
Each student must present a written report about the lab experience.
Teaching tools
TV projector, overhead projector, PC and cell culture
laboratory
Office hours
See the website of Gian Paolo Bagnara